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Questions related to Mouse Models
I am trying to find other animal model study other than tail bleeding assay to analyze pharmacodynamics in hemophilia A mouse model (FVIII KO). I am aware of transection model, however I want to...
11 November 2016 9,335 1 View
We are doing an experiment where mice must eat a highly preferred food, which has been manipulated to have an unpleasant taste. We planned to use quinine but it may not arrive in time for...
11 November 2016 1,201 3 View
Does high dosages of glutamic acid and apartate may kill mice during exercise?
11 November 2016 6,572 2 View
Hello I'm trying to collect the left ventricle from a mouse heart and i have zero experience in that. i tried to look for videos and publication and i didn't find any useful ones could any one...
11 November 2016 5,870 2 View
Hello I'm Yerin Kim struggling with mouse wound healing research. I've just made full excisional wounds on mouse back and put Tegaderm film(3m) to protect it from infections. And I found that...
11 November 2016 603 10 View
Hi, I'm having trouble trying to stain mouse IL-4 from Th2 cells polarized in vitro.I've been purifying CD4+ cells from BABL/c spleens with magnetic beads and culturing them in CD3/CD28 pre-coated...
11 November 2016 8,662 8 View
Does anyone treat mice (B6) with tamoxifen to mimic ovarian ablation (ovariectomy)? How much and how long do you treat the mice? Thanks.
11 November 2016 2,860 2 View
I've recently done an RNA extraction from mouse liver progenitor cells- on running my RNA samples on a gel, I noticed a distinct band above the 28S. Any ideas on what it may be? I don't think it's...
11 November 2016 941 3 View
As you know whole eye is a harder/more solid tissue, and we only have micro tissue homogenizer in our lab (with polypropylene pestles) Is it possible to homogenize mouse whole eye with it?
11 November 2016 1,200 1 View
Hi, everyone. I am working on using inducible CreERT2 system to knock out (down) target gene. I did extensive experiments to validate it. I found 2.2 mg/per adult mouse with 4 injections could...
11 November 2016 9,434 5 View
Need to have an indicator of when the dam will have her pups within a 1-2 day window to facilitate cesarean rederivation.
11 November 2016 5,742 6 View
I am injecting an AAV into the hippocampus of adult mice that upon infection, lead to expression of a protein only in neurons. While I am successful in getting this protein expressed in just my...
11 November 2016 8,986 7 View
I have found many different protocols to see amyloid-beta plaques using Thioflavine S and more or less all of them have the same steps: deparaffinization/hydration, incubate with 1% Thioflavine S...
11 November 2016 8,544 6 View
I notice that most people use 4-OH tamoxifen for IP administration and tamoxifen citrate for oral administration. Tamoxifen citrate needs to be metabolised by the liver to be effective so I assume...
11 November 2016 644 5 View
To ensure maximum number of pups with congenital diaphragmatic hernia
11 November 2016 2,539 3 View
does anyone have a detailed protocol for isolation of SNc from mice brain? and which dye is used for stain Dopaminergic neuron cells?
11 November 2016 6,940 3 View
I could not find papers dealing specifically with that subject. I found some with rats, but none with mice. Specially regarding ladder climbing protocols in high fat fed mice. Does anyone know of...
11 November 2016 3,990 0 View
I am Working on Primary Neural Cell culture from SVZ of the Postnatal Mice Can anybody Help me to Provide Some guidance how to Precisely cut this part. and How to recognize SVZ in Brain of...
10 October 2016 5,932 1 View
pancreatic islets
10 October 2016 1,642 1 View
We are wanting to administer a pH1.8 (or 0.015849M) solution of HCl to an anaesthetised mouse lung via a tracheal tube. We are wanting to do this either with standard Thermo needles and syringes,...
10 October 2016 9,230 1 View
We have a breast cancer mouse model from which we want to extract the tumour, dissociate in single cells and then remove all non-tumour cells to have a pure population of tumour cells to inject in...
10 October 2016 2,269 2 View
Hi guys, I am working with the mtb-infected mouse model. and i am thinking about whether we can monitor the bacterial burden with mouse blood. the current way we do for the bacterial burden...
10 October 2016 6,561 4 View
Would you please let me know what would be the best way to measure gluconeogenesis in-vivo in mice, I would appreciate.
10 October 2016 2,288 2 View
What are the parameters most required? For liver is there anything more than AP, ALT and AST? And for kidney anything more than Creatinine and Uric acid?
10 October 2016 2,327 1 View