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Questions related to Mouse Models
Does anyone has the same issue of the relative low survival rate of the obese mice after surgery? My mice were anesthetized by ketamine and xyline. I also give the pain killer (Buprenophine)...
02 February 2017 8,116 6 View
Hello, I've a general question as literature is inconsistant: Who has experience with melanoma in vivo models and can help me? I tried to inject 200 thousand cells s.c w/o Matrigel and started...
02 February 2017 6,498 8 View
Recently, I am trying to isolate islets from db/db mice. I use the same protocol I regularly use for islets isolation from mice but I can't get any islets or very few pale islets attached to...
02 February 2017 5,940 7 View
To everyone working with progesterone receptor in rodent brain the rabbit anti-human progesterone receptor antibody from DAKO is sadly not produced anymore...Thus, I am searching for an...
02 February 2017 4,954 0 View
we are trying to infect mice (BALB/c) with HSV-1F. but no visible changes are there on the skin like mentioned in literature (blisters on the area of infection). We administered intradermally and...
02 February 2017 2,122 5 View
I typically add bicarbonate until the pH of the solution is 7.4, although sometimes it is slightly above.
01 January 2017 4,848 0 View
Does anyone have a good protocol for genotyping APC flox mice from Jax (https://www.jax.org/strain/009045) C57BL/6-Apctm1Tyj/J? I am also looking for a std. PCR assay since the genotyping protocol...
01 January 2017 6,438 2 View
Hi everyone, I have a question please. We have our BMDM ready, but it did not respond LPS (100ng/ml)and IL4(25 ng/ml) very well. I wonder if anyone have any suggestions or experience on the...
01 January 2017 1,187 2 View
especially for exercise training
01 January 2017 1,769 7 View
Hi there, Would like to ask some opinions from you all out there. Lets say I have a mouse dosing study to study the effect of drugs A and B in tumor bearing mice. Therefore, my treatment groups...
01 January 2017 8,779 3 View
Can I use the Soxhlet extraction method for this? If yes, how do I go about it?
01 January 2017 6,463 4 View
I´m searching for a protocol which can help me evaluate blood vessel permeability in mice who have been submited to a skin ulceration protocol (creation of excisional wounds). I already know that...
01 January 2017 745 1 View
Does anyone have a successful protocol for immunohistochemical staining of mouse toes? In particular, which fixation technique was used, was decalcification necessary, and were frozen sections or...
01 January 2017 6,129 6 View
I am working on a project trying to detect a GFP in intestine stem cells. I tried Abcan 290 GFP antiboday staining but failed. It works once for endogenouse GFP signal on cryosection for the same...
01 January 2017 6,723 2 View
Hi, My lab have GFP transgenic mouse. GFP-transgenic mouse tissues 'Paraffin block' I did IHC and I could detect gfp positive cell. However, I tried GFP-transgenic mouse IF. I could look...
01 January 2017 6,116 2 View
Hi, I am currently trying to establish a model of intestinal ischaemia/reperfusion injury in mice, in my lab, with a goal to producing local injury and acute lung injury as a result. However, I am...
01 January 2017 5,292 2 View
Dear all, I would like to determine creatine kinase blood concentration in mice after exercise. In human, the peak blood concentration is around 12-24 hours after the exercise. After the exercise...
01 January 2017 3,369 0 View
What are the toxic levels in I.P or I.M injections for mice? I know there is a range, and I think I even read a paper stating it plainly, but I seem to misplace it, and can't find it. Does any one...
12 December 2016 8,379 1 View
Hello all, I'm interested in testing the cardiotoxicity of different drugs of interest using a Ca2+ influx assay, specifically the Molecular Devices EarlyTox cardiotoxicity kit that uses fura-2...
12 December 2016 7,190 2 View
and also in how many minutes/seconds it produces the seizures in mice?
12 December 2016 4,092 2 View
I would like to feed STZ-injected mice (type 2 diabetes) on cholesterol diet for 28 weeks. Is this period enough to induce atherosclerosis? since wild type mice are resistant to atherosclerosis
12 December 2016 1,905 5 View
Hello, I am trying to make phototrombotic ischemic stroke mouse model. The procedure is 1. Intraperitoneal injection of Rose bengal 50ug/mouse weight (g) 2. After 5 min, switch on the cold light...
12 December 2016 3,072 5 View
When we dissected our mice after treatment we found that the skeletal muscle in the treatment group were 'dry' and 'less fleshy' as compared to placebo.The mice were otherwise active and healthy...
11 November 2016 6,756 13 View
Now, I vaguely know that if an infertile male mouse mates with a female she becomes pseudopregnant but what are the characteristic features of a pseudeopregnant female? how do you identify...
11 November 2016 2,948 1 View