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Questions related to Mouse Models
In Alzheimer's disease mouse model behavior test (open field, Y-maze, water maze etc.), is there behavior difference between male and female 5xFAD mice which affect data result and get...
01 January 2018 3,019 1 View
Hi Everyone, I am trying to overexpress a gene of my interest specific to cardiac fibroblasts. Does anyone know a selective virus targetting fibroblasts in-vivo mouse model? Thanks
01 January 2018 3,468 1 View
In the KrasLSL-G12D/+;Pdx1-Cre PDAC mouse model, at which time does metastasis start to form in lung and liver? And at what frequency?
01 January 2018 8,814 2 View
i need to check calcium overload in adult cardiomyocytes from AMI mouse model, kindly share the protocol and relevant literature. Thanks
01 January 2018 8,833 2 View
Now i am testing the therapeutic effect of a new humanized antibody in mouse model, and the original rodent antibody has been proved to be effective in B6 mice. Should i use nude mice for this...
01 January 2018 9,936 2 View
I am going to induce acute myocardial infarction in rats by LAD ligation. I want to know what protocol is most suitable for getting to the heart. Thoracotomy or lateral thoracotomy? I would be...
12 December 2017 4,650 13 View
I'm currently working on a mouse model that becomes obese and glucose intolerant at the age of 7 months when fed standard chow. Since I'm primarily a reproductive biologist, I have a naive...
12 December 2017 9,704 3 View
When I did the qPCR experiment using the TLR4 assay on spinal cord RNA of TLR4 KO mouse, the TLR4 mRNA was measurable. I did the genotyping of this tissue sample, and it was a homozygous clean KO...
12 December 2017 2,617 3 View
I am going to induce acute myocardial infarction in rats by LAD ligation. As you know, the mortality rate in this model is usually high. What should I do to reduce the casualties? I would be very...
12 December 2017 9,866 26 View
Hi, I performed several glucose tolerance tests (GTT) to assess glucose tolerance in mutant mice compared to WT mice. Each test was done on 5-6 mutant mice and 5-6 WT mice at the same time. All...
11 November 2017 861 4 View
I am working on a prostate cancer mouse model with PC3 cells in nude mice. Does anyone have experience with the best number of cells for inoculation and if addition of matrigel is necessary?
11 November 2017 4,414 3 View
I am looking for a Housekeeping gene that has a CRE promoter to use as internal control (mouse model) for my RT-PCR, Any suggestions?
11 November 2017 6,401 1 View
Dear colleagues, I am going to induce diabetes in C57/BL6 mice using a high dose of (150 mg/Kg) STZ. I should be able to keep them for 16 weeks after induction of diabetes. May my potential dose...
11 November 2017 6,059 21 View
Dear colleagues, I am going to induce acute myocardial infarction in rats by LAD ligation. We do not have access to ECHO and ECG to confirm the MI model. Does the observation of damaged area...
11 November 2017 6,253 6 View
Astrocyte dysfunction in the "mouse brain during sleep deprivation" is often taken as the explanatory model of "what goes on in the human brain during sleep deprivation"? From a research...
11 November 2017 8,042 0 View
develop knockut mouse
10 October 2017 3,371 3 View
What do we know about the interaction of cancer cells with murine host cells? And how are murine host cells replaced by cancer cells?
10 October 2017 6,996 8 View
which the stage/level of clinical trails are mouse models used in？ In the U.S., drug development has pre-clinic, first, second, third stage. Which the stage that required to use mouse models to...
10 October 2017 1,906 5 View
I have mutation calls for mouse genomes and i want to calculate the mutational signatures from these calls, I know how to do it for human genome using deconstructSig but i never came across a...
10 October 2017 5,591 1 View
I would like to test the effect of a particular gene on fertility of males and females. I m planning to use a knock out mouse model and a cell line model. What are the appropriate cell lines used...
10 October 2017 8,898 0 View
1. Explain what is meant by the term “knockout mouse”. 2. Describe how knockout mice are made overall strategy core method: traditional ways: TELENs, ZFNs, .... new ways:...
10 October 2017 2,821 1 View
How informative is the use of mouse model for human gut microbiota research? What are the pitfalls and how far we are in mimicking the mouse with human gut? thanks
10 October 2017 1,236 3 View
I have H&E stained slides of lungs from mouse models of lung cancer. I was wondering if I can calculate tumour volume or area from the H&E stained slides. Thank you. Vrushali
09 September 2017 1,106 1 View
we are administering radioimmunotherapy in HER2 positive breast cancer mouse model. I was wondering if there are any useful bio markers to look at certain point post treatment. Ki-67...
09 September 2017 1,531 1 View