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Questions related to Mouse Models
Hello So I have mutant mice that misproject axons in the spinal cord. Essentially, axons enter a territory that they are not supposed to be in. I want to image the spinal cord as a Z-stack. I get...
10 October 2016 9,149 2 View
How to convert male C57Bl/6J mice (20-27 grams in weight) dose to male Wistar rat (200-250 grams in weight) dose for attenuated live bacteria? (in colony-forming units, intraperitoneal injection)
10 October 2016 7,465 3 View
I have been using a 40% Dextran gradient centrifugation protocol with HBSS to isolate mouse capillaries (from cortical homogenate) but have not found any of my pericyte or smooth muscle cell...
10 October 2016 4,883 12 View
I'm looking to administer estriol over a long period of time (like more than a year). Multiple pellet implantations is not really feasible because it would be really bad for the mice. Also IP...
10 October 2016 7,675 5 View
Hello Respected Members I want to know that, what could be the best way to check (developmental progress) the mitosis and meiosis during spermatogenesis (in-vitro), Gene expression,...
10 October 2016 4,601 4 View
I'm looking to run a series of experiments involving IHC and protein quantification (WB or ELISA) on mouse brain. I was wondering if it was possible to flash freeze half the brain for IHC, and...
10 October 2016 6,933 4 View
Hello everybody, I'm trying to isolate and culture neonatal cardiomyocytes from mice using the protocol published on Jove and titled "Isolation and culture of Neonatal Mouse...
10 October 2016 3,993 2 View
In the Paxinos & Watson mouse atlas, each page/slice has a coordinate grid and is referenced to some landmark (for coronal, A/P relative to bregma). The reference mouse atlas from the Allen...
10 October 2016 1,966 10 View
Does anyone know a good mouse model of neonatal exposure to maternal obesity?
10 October 2016 9,403 2 View
I am trying to isolate GFP +ve microglia from adult mouse brain (1-2mo). I have been using a modified protocol from Derecki (2012), but using a Percoll gradient for myelin removal. My problem is...
10 October 2016 5,994 4 View
I am culturing embryonic neural progenitors cell from mice from E11.5 time pregnant mice. And differentiating them into neurons by removal of bFGF but after doing qPCR of cells without bFGF and...
10 October 2016 6,206 4 View
I have some mouse heart tissue embedded in OCT. Usually we take cryosections but we have decided to try and make thicker sections with a vibratome. I was wondering if anyone has experience making...
10 October 2016 2,154 3 View
What should be the endpoint (in the context of tumor size) of an experiment in which the animals are injected with CT26 cells? The idea is to measure the tumor until the endpoint, at which point...
10 October 2016 7,205 4 View
I am working on the assessment of the efficacy of several new vitamin D analogus in treating postmenopausal osteoporosis in rat model. Postmenopausal osteoporosis was induced by performing...
09 September 2016 5,584 3 View
I've seen different recording locations when doing nerve conduction studies on mice sciatic nerves. Some are the gastroc, TA, and the foot. Is any one better than the others, less prone to error,...
09 September 2016 5,008 2 View
Hi! This protocol has been used in my lab to handle healthy rat liver samples (see below). But now, i'm going to start a project for quantifying membrane proteins in healthy and diseased human...
09 September 2016 3,888 2 View
I had prepared mouse adult fibroblasts from Wild type and Knock-out line. They were proliferating very well. I immortilzed them with SV40 T large antigen vector at passage 4 and split them till 10...
09 September 2016 8,986 2 View
Currently, I’m working with model of gastric ulcer induced by EtOH or NSAIDs (diclofenac). I use ImageJ software for macroscopic scoring to compare the ulcer area. Unfortunately, I couldn’t find...
09 September 2016 4,598 2 View
We want to label the blood vessels in rats to check under the in vivo microscope, and follow one week after labeling. We have used FITC-dextran 2000S but there was leakage into the parenchyma,...
09 September 2016 4,604 7 View
In my experiments the tumor injection and growth is subcutaneous in the neck. Is it OK to locate the micro-chip (7mm*1.5mm) also in the neck, at separate sides as much as possible? Is it...
09 September 2016 3,810 2 View
I need to conduct open field and morris water maze on groups of mice. If I do open field in the morning, can I do morris water maze in the afternoon, or will that interfere? Thanks!
09 September 2016 1,969 3 View
I have mouse cortical astrocytes which have already been passaged twice and are in healthy condition but want to passage them again for my experiment. Is it safe to do ? And do I need to use the...
09 September 2016 3,068 5 View
Our lab is going to purchase some mouse embryo from EMMA (European Mouse Mutation Archive). In order to fill out the MTA, we need to provide the information of the provider and administrator to...
09 September 2016 7,965 0 View
Hello everyone!!! I have gone through a protocol for the estimation of 11 beta HSD1 from rat by ELIZA kit method, in that they mentioned that can use tissue homogenates and other...
09 September 2016 9,900 1 View