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Questions related to Mouse Models
We want to measure dystrophin restoration after a treatment in DMD-KO mouse model by immuno or western.
02 February 2021 5,012 2 View
Hello, I am a first-year Ph.D. student and I would like to investigate factors regulating ACE2 gene expression using a mouse model, but I am unsure which mice to use. Which mice would you...
14 December 2020 3,135 2 View
Hello, I am a first year PhD student and I am working on Cerebral amyloid angiopathy (CAA). I would like to investigate the presence of my proteins of interest in a CAA/Alzheimer's disease mouse...
06 December 2020 1,142 3 View
I am trying to make syngeneic mouse model for TNBC for that I need 4T1 cell line. It is quite expansive commercially. So if anyone knows from where to get it or if someone can share it will truly...
16 October 2020 2,126 2 View
For those working with mouse models of circadian rhythm disorders, or using changes in the light-dark cycle to induce circadian disruptions, what is (are) the best options for cabinets during the...
08 October 2020 1,479 1 View
what is the best mouse model for lung cancer study? No Knockout Mice We Have Simple Strain?
28 September 2020 5,930 1 View
I'm trying to isolate left ventricular myocytes from wildtype C57BL/6 mice. Currently the isolation step seems to be going okay - usually I get >50% viability of cells (although this is...
17 September 2020 4,441 3 View
i want a xenograft MDA MB231 cell line animal model. can ER, PR and HER2 negative model be prepared by using an immunodeficient mouse model eg. BALB mice and which chemical can be used for this...
18 August 2020 7,418 3 View
My recent mouse model found a hematological tumor, but the onset of mice is fast and there are few blood stem progenitor cells. It is difficult to judge tumor cells with a second transplant. Is...
28 July 2020 9,849 1 View
I have been reading about A53T aSyn genetic PD mouse model, and I realized that 99% of all of them, are based on the expression of human alpha-synuclein (wild type or mutant) in the mouse. I have...
27 March 2020 4,169 3 View
I am getting difficulty in getting cell marked distinctly. I am using mouse model with the following protocol: Pre exposure habituation in the chamber - 20 minutes Exposure - 15 minutes Post...
11 March 2020 5,877 3 View
Hi, We don't have radiator on our site, but we want to study human tumor cell line in xenograft mouse model. Does anyone know any mouse strain (besides nude mice) or alternative way to do it ?...
23 January 2020 4,938 1 View
Hi, I have some problems with carbon tetrachloride (CCl4) -induced mouse model of liver fibrosis, and would like to ask is any of you has experience in troubleshooting this model. I run model on...
16 January 2020 5,877 6 View
since apomorphine is not availble, and pramipexole and apomorphine are both dopamine receptor agonists, so I'm wondering if I can use other dopamine receptor agonists to verify the rotation test...
09 January 2020 278 3 View
01 January 2020 5,473 2 View
01 January 2020 9,498 3 View
I have found variants in a gene in which I have done in-silico analysis for and it is thought to be disease causing. If I want to study these variants more and see their effect, would it be...
01 January 2020 7,965 3 View
I am interested in exploring the contribution of obesity in murine wound healing without the characteristic hyperglycemia of diabetic mice. I have considered the use of an obese diabetic mouse...
11 November 2019 9,605 2 View
As nude mice still has Bcells,NK cells and DC cells. Will K562 cell lines survive in Balbc nude mice to create a xenograft model. There are very few publication who used this strategy to test...
11 November 2019 8,592 2 View
Im working on a caloric restriction (CR) mouse model. The mice undergo 16 hours of fasting, where water is available. I have moved on to GC-1 cell line. What would be the most appropriate method...
11 November 2019 5,277 1 View
Sample： splenocytes of mouse model I have been using MACS negative isolation method for 1.5 years, and it worked fine. usually the yield will be ~1M/spleen, but in recent half year, I kept...
30 October 2019 7,230 1 View
I am searching for a lab for my graduate studies that is aimed at understanding the molecular workings of depression/schizophrenia/anxiety. Preferably working with mouse models and in Germany.
21 October 2019 6,662 1 View
10 October 2019 7,728 1 View
Hello, I have been looking at the literature and am struggling to find the mechanism(s) whereby mouse models of large intestinal inflammation and damage (e.g. colitis) induce a reduction in large...
10 October 2019 6,265 2 View