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Questions related to Mouse Models
I have rats that will be 600+grams at time of testing in the Montoya staircase test, all the units i have seen for sale are most suited to rats upto 300g with some stating have been used for rats...
09 September 2016 2,829 1 View
Has anyone used NF639 cells to grow sub cutaneous tumors in FVB or Balb/C mice?
09 September 2016 2,020 4 View
To those who have experience with microglia sorting from glioma mouse model. Could you please share how many CD11b+ cells were you able to sort from one tumor bearing hemisphere (flow cytometry or...
09 September 2016 4,732 1 View
I'm looking for a good protocol for sectioning frozen undecalcified rat bone. My peptide of interest is very small and vulnerable to degradation (it will probably not survive decalcification or...
09 September 2016 9,615 3 View
I need to inject 5X10^7pfu vaccinia virus into one mouse. So I have to produce more than 10^9 pfu vaccinia virus. Now I can only produce 10^8 pfu (1ml) virus from 10X150mm tissue plates using...
09 September 2016 981 1 View
We are doing behavioral tests on rats and mice on risk taking by using predator odors. So, we want to do the trials during the night as starving them during the nigh hours gave us significant...
09 September 2016 3,435 1 View
I have to choose a glucometer for my research on mice. Many of those reported on publication are not available in Poland. I would like to know if some has experience with Accuchek Active or...
09 September 2016 5,061 4 View
Hi everyone, I plan to engraft 1 x 10^6 MCF-7 cells into the mammary fat pad of female athymic (nude) mice. In your experience, how long will it take for the MCF-7 tumor to reach 200 mm^3? If you...
09 September 2016 7,519 1 View
Hi everyone, I hope this email finds you well. I have two questions about breast tumor xenografts, which I hope to find answers from you. 1. To assess metastasis from tumor xenografts, what...
09 September 2016 9,866 0 View
I know this type of assay is better done with a rat however our circumstances left us using a mouse model. That being said, its pretty difficult to get this aortic ring to respond to much of...
08 August 2016 384 1 View
I am working on the effect of fenofibrate on the 4T1 breast cancer metastasis by using Balb/c mice as the animal model ... but I have a problem in how I could give the required dose of fenofibrate...
08 August 2016 5,555 1 View
Hello, I have some general questions/curiousities: I need to inject s.c. a drug that is first diluted in DMSO and than in oil (I use corn oil). Final DMSO conc is 5%. The total volume is 200µL,...
08 August 2016 5,370 2 View
I have a mouse model that expresses modified Cre recombinase, so that only upon tamoxifen treatment of the animals they express Cre, thus KO of floxed gene can be induced. I want to isolate the...
08 August 2016 2,686 2 View
Hello. I've posted a question before, however, it may have been a bit wordy, so i'll try to be more brief here. We are working with 2.5mm mouse thoracic aortic rings in a 25mL Radnoti tissue...
08 August 2016 2,715 5 View
Hello. I'm currently troubleshooting an mouse aortic ring assay and I'm just trying to get the ring to respond to anything. When I add the positive control (phenylephrine in this case, although we...
08 August 2016 1,139 2 View
Hi, I'm currently culturing E11.5 mouse brains in organotypic slice cultures to look at the effect of a drug on cortex formation. I am dissecting out the brains, embedding them in 4% agarose,...
08 August 2016 3,745 6 View
I have some mutant mice which show elevated blood glucose levels as compared to controls. So I just wanted to take valuable opinions of all of you about which factors relating mainly to beta-cells...
08 August 2016 3,335 6 View
What is the maximum amount of mice that can I intratracheally infect with a 50 uL stock of adenovirus with a titer 2.4E10 PFU/Ml. Each mice will be injected with a final titer of 2.5E7 PFU/mL.
08 August 2016 6,544 0 View
I usually dissolve Dox HCl in sterile NaCl 0.9%. The dose of 5 mg/kg IP once weekly seems too toxic in my experiments, even though this dose was used and tolerated in multiple published articles....
08 August 2016 3,561 3 View
I’m planning to investigate induced liver cirrhosis in rats, what is the best chemical for inducing liver cirrhosis in Rats, and what doses should be used and for how long? I know about ccl4 and...
08 August 2016 6,472 0 View
I have a difficulty to understand outcome of following experiment. Experiment outline Mouse Wild type (control) Heterozygous mouse (conventional knock-out (Gene+/-)) Sample RNA from Total bone...
08 August 2016 4,075 4 View
Dear Researchers, I am working on a protocol to study the effects of a compound on sperm motility. 1) I will be collecting the Cauda epididymis at the animal facility and will be taking the tissue...
08 August 2016 2,864 6 View
Dear all, I am starting a new project and I need to buy a glucometer, for measurement of blood glucose during GTT. As this is a new procedure for me and we do not have in my lab, I would like to...
08 August 2016 7,322 4 View
Hello, I injected MCF7 cells in NOD SCID IL 2 RG null mice, after transplanting an estrogen releasing pellet (not between the shoulders on the back, but on the side). No matrigel, just cells...
08 August 2016 958 3 View