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Questions related to Mouse Models
I would like to inhibit HSP70 in vivo (mouse) with PES or anti-HSP70 antibody. Does anyone know the right amount to use or alternative methods to inhibit HSP70 in vivo? Thanks
08 August 2017 2,818 0 View
Hello, I am doing a study on lineage tracing using a cell-type specific reporter crossed with a CreER line. I am looking to study these at e17.5 and am curious if anyone has recommendations for...
08 August 2017 3,130 3 View
I am trying to induce acute kidney injury in mice (male DBA/2j, 20-25 gr) by using gentamicin (80 mg/2ml). mice were treated with gentamicin (200 mg/Kg, i.p.) for 10 consecutive days. I have...
08 August 2017 6,508 5 View
Our lab, in Brazil, recently read the paper "Anti-inflammatory mechanism of lonchocarpine in LPS- or poly(I:C)-induced neuroinflammation". The authors say "Lonchocarpine (50 mg/kg) was dissolved...
07 July 2017 7,989 2 View
For some reasons, I need to use C57 or DBA mice with impaired immune system for organ transplant from other species such as Rat or monkey. SCID/NOD mice, however, can not be used in my exprements....
07 July 2017 8,365 4 View
I am wondering if anyone could help on that issue. I'm working with CBA/J mice and I have planned to feed them antibiotics in water. I have just found out that there is 500mg of ethanol in the...
07 July 2017 3,161 2 View
Read 8 answers by scientists to the question asked by Ratish Raman on Jun 27, 2017
06 June 2017 9,912 8 View
Alzheimer's disease
06 June 2017 9,182 1 View
To obtain the serum from a mouse blood sample I perform this protocol: 1. Obtain the sample withouth any anticoagulant and transfer to a strile empty tube. 2. Leave the tube in a standing...
05 May 2017 9,471 4 View
I have engineered a tissue specific knock-in mouse model and I need a mechanism to label these tissues. I tried a commercially available Flp-inducible reporter animal, but it also had an...
05 May 2017 3,937 2 View
Hi, I am planning to start an animal experiment investigating time course of neurometabolic cascade post mild TBI (traumatic brain injury). The problematic part is the animal model. Unfortunately,...
05 May 2017 8,948 1 View
I'm looking for a serum-free long-term (> 4 d) culture system for mouse granulosa cells. Protocols exist for sheep and cattle, but I cannot find one for mouse. Local colleagues simply say that...
05 May 2017 9,032 5 View
Hi everyone, I am going to use DSS to induce acute colitis, and check the immune response, including innate immune cell and adptive immune cells, here the question is that: What is the...
17 April 2017 6,322 3 View
My mouse embryonic stem cell line are not working. They are not able to attach to the surface of coated dishes ( I have tried both Geltrex and 0.5% gelatin) remain in suspension. I am using ESGRO...
04 April 2017 2,493 12 View
04 April 2017 5,281 3 View
I'm interested in anesthetic-induced neurotoxicity and performing the experiment. In my experience, during anesthesia with isoflurane, respiration of mice is suppressed, and then PCO2 level...
04 April 2017 585 0 View
i use ketamine/xylazine (110mg/kg and 25mg/kg) for sedation, buprenorphine administered at 0.1mg/kg before surgery and body temp maintained at 37*C during surgery. Most of the mice are dead within...
03 March 2017 4,628 1 View
I have two different groups: my treated group and my control group. Unfortunately, I can not see any difference between my groups in terms of discerning the novel object. However, I have found...
03 March 2017 338 7 View
Hi all, I am trying to test bone marrow vascular permeability in couple mouse models. In order to confirm the change, I would like to have a positive control with increased vascular permeability....
03 March 2017 3,444 2 View
I tried to measure my drug sunitinib 0.1mg/kg (0.07mM)samples in mice plasma and used an internal stranded verapamil 50nM and made a calibration standreds from 1nM to 3000nM but the problem is in...
03 March 2017 7,588 3 View
Hi, Can anyone tell me if there's a specific range for the immobility time/percentage that 'normal' adult C57BL6 display in the Porsolts Forced Swim Test (5 minutes analysis, only one trial of the...
03 March 2017 3,358 3 View
Dear Sir/ Madam, We had conducted a study on memory impairment process in 3xTg AD (3xTg Alzheimer's disease) mice model. We observed significant memory impairment in these mice at their age of 24...
03 March 2017 4,541 5 View
What is the recommended dose of insulin to perform insulin tolerance test in C57BL/6JUnib mice (fed on chow or high-fat diet)? I have found 0.1 to 10 UI/kg in the literature. Which is an adequate...
02 February 2017 911 3 View
Dear colleagues! is it necessary to breed the female with vasectomised male for pesudopregnancy. As breeding objective is ovulation induction and preparation of uterus for carrying pregnancy. But...
02 February 2017 5,105 3 View