What is the best way to measure gluconeogenesis in-vivo in mice?

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Do you have suggestions for feed formulation software?

Hi, I am considering buying a feed formulation software that could be used for teaching as well as animal experiments for both ruminants and monogastric species. Do you have any suggestions for...

11 December 2018 5,482 34 View

How to calculate area under curve (AUC) in R?

I would appreciate if you share R codes, information, suggestions etc. regarding calculation of area under curve in R for time-series data. Thanks

08 September 2017 2,903 7 View

Is Picro-Sirius Red staining also applicable to frozen sections?

Picro-Sirius red staining has most frequently been used on paraffin sections and I was wondering if it is possible to use this for cryosections as well. I would appreciate you comments,...

08 September 2017 8,777 2 View

How to compare histograms from different groups in R?

Hi there, Will you share how to compare histograms (frequency distribution) from different groups in R? Would appreciate,

07 August 2017 6,190 24 View

Independent t-test with covariates?

Hi all, I have two independent groups I want to test whether their values differ significantly for a number of parameters studied. Based on my statistical knowledge from early university, the...

01 February 2017 9,931 13 View

How to use lsmeans to make interaction plots in R?

Hello there, I would appreciate if you could provide some tips on how to use lsmeans to make interaction plots in R. Thanks alot

01 February 2017 3,529 11 View

How to deal with two repeated variables (nested) in lme R?

Hi I have a response variable (response) measured hourly 48 hours (hour) and repeated this again for 5 weeks (week). I would appreciate if you suggest how would you tell this to lme in R with...

11 December 2016 2,459 5 View

In a repeated mixed model analysis, the repeated variables such as time or week etc. are treated as a factor or continuous variable in the model?

Hello there, I am performing repeated measures analysis where I need to look at changes in blood parameters over a period of time (lets say week). So, should I use week as a factor or continuous...

09 October 2016 5,711 10 View

What could the best way to preserve mice pancreas to obtain good RNA yield for qPCR?

Hello everyone, I had had a difficulty in obtaining good RNA yield from sheep pancreas samples in the past. This time I am going to try with mice samples. Would you please suggest effective ways...

08 September 2016 7,618 3 View

Glucose tolerance test in mice: Do you prepare 20% glucose solution in NaCl solution or in water?

I would appreciate if you can answer whether you prepare glucose solution for injection in mice in NaCl solution or just in water and why!

07 August 2016 7,314 9 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Enhancing Critical Thinking Skills for Slow Learners: A Review of Empirical Studies?

to identify themes in question with APA style references

07 August 2024 2,239 5 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View