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Questions related to Mouse Models
I have frozen mice livers, pancreas and skeletal muscle. The blood sample obtained at the time of sacrificing the animals was just enough for complete lipid profile. i need to get an idea of...
08 August 2016 2,623 7 View
I need to know the normal range of BMD in C57BL/6 mice. what's the critria for mice osteoporosis?
08 August 2016 5,300 2 View
recently I came across this paper: Genome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis It says in 67,405 single-guide RNAs (sgRNAs) there are 1000 control gRNAs. And I want...
08 August 2016 3,254 0 View
I would like to know if I can use these combination of markers to label mouse spleen DCs, instead of using the pan-DC gate on CD11c.
08 August 2016 8,067 5 View
Hi. I have recently purchase the alpha-TC9 rodent glucagonoma from ATCC. According to ATCC protocol, the media should contain 0.02% BSA. Can anyone help to recommend a suitable brand for the BSA...
08 August 2016 8,696 1 View
we did plasma measurement of cho and tri. in our mouse samples, now we would like to measure direct level in the fresh tissues, does anyone know where we could do this around montreal area, or any...
13 July 2016 6,293 5 View
I want to use it to knockout one gene in mice.
07 July 2016 5,710 0 View
I just want to know whether does make a sense normalize the absolute result of electronic von Frey (in grams), with the animal’s weight. Indeed, our doubt is; as von Frey is a mechanic threshold...
07 July 2016 4,421 1 View
Dear all, I'm checking the effects of the combination between two compounds in an asthma mouse model. During metabolism, these two compounds shared some similar cytochromes, such as CYP2C9,...
07 July 2016 9,714 3 View
Hi guys, How do you mount the dissected hippocampus onto specimen disc for horizontal sectioning? Currently, I am trying to set up a rig to recording field EPSPs using mouse horizontal slices....
07 July 2016 7,179 4 View
I was looking into different literature and I want to know if there is a guideline for fasting glucose level for OGTT glucose level. It seems like mice with >8mM (fasting) and >7.8mM (OGTT)...
07 July 2016 9,663 6 View
Dear all, I want to inject the FACS sorted iNKT cells into the mice liver. I am thinking of injecting retro-orbitally but I don't know how many cells will eventually reach the liver. Moreover, I...
07 July 2016 1,452 2 View
This ultradian cycle is used to induce depression in mice without altering their sleep amount. Unfortunately it's not possible to set up this T7 cycle on a classical 24h timer clock placed in my...
07 July 2016 2,944 4 View
I have rats lesioned with 6-ohda in the medial forebrain bundle, that degrades dopaminergic projection neurons to the striatum. I would like to quantify the expression on D2 receptor on the...
07 July 2016 6,300 1 View
Hey everyone, i am trying here to stain mouse spinal cord with parvalbumin(mouse origin), from swant, dilution 1:5000 with alexa fluor 647 or 633. The problem is that that the staining is very...
07 July 2016 7,457 3 View
07 July 2016 10,012 4 View
Does anybody have experience with transtympanic / intratympanic injections in mice/rats? I want to learn and use the method. Preferably in Germany. Thank you in advance
07 July 2016 6,496 0 View
I need a way to distinguish homozygous Col2acre mutants from heterozygous Col2acre after they have been bred with a flox/flox mouse model.
07 July 2016 1,074 2 View
Stereotaxic surgery electrode implantation Mostly it came off around 20 days with the implanted electrode, which is the issue i am facing right now ,can anyone help me with this issue?
07 July 2016 2,406 4 View
hippocampus- astrocyte isolation
07 July 2016 9,660 4 View
Hi everyone! I am preparing to do the efficacy of human NK cells in Non-small cell lung cancer (NSCLC) Scid mouse model, and now I am finding which kind of NSCLC cell lines are sensitive to NK...
07 July 2016 7,683 2 View
Hello I'm looking for transcription factor resource that would give a list of transcription factors and their corresponding target gene. The species I'm mostly interested in are Mouse.
07 July 2016 6,992 1 View
I am running a PCR to determine if my mice are knock outs or wild type for an ephrin allele. I just took over a mouse colony. The PCR for the parents works well and gives accurate, strong bands....
07 July 2016 3,672 4 View
What features of cell lines should be taken into consideration when I want to grow a solid tumor inside mouse? Can I inject any kind of cancer cells into nude mice to gain a tumor? and what...
07 July 2016 9,943 3 View