Hello
I'm trying to collect the left ventricle from a mouse heart and i have zero experience in that.
i tried to look for videos and publication and i didn't find any useful ones
could any one suggest a good video or a protocol of how to distinguish the mouse left ventricle and what are the tools used to collect it ?
thank you
The left ventricle wall is larger than rigth ventricle. And a good way to indentify the left ventricle is following the aorta.
Hello, this is not really a protocol, but a basic HowTo...
Left ventricle is the biggest part of the heart, so you can collect it in a "just remove everything else" way. When you have the heart, take small scissors and cut away all the small parts that are "hanging" from the heart (if you can lift it with the tip of your scissors, then it should go away). That way you should get rid of the aorta and both atria and you will get firm egg-shaped thing. One side will be a bit softer and bulging. That's the right ventricle.
From the where-aorta-was side insert scissors and cut down so the right ventricle will open. Remove that and you have only left ventricle left.
Small curved scissors are sufficient, if available spring-type micro scissors are better for removing the tiny bits. For basic anatomy-learning purposes, some people found it helpful to leave the heart for several hours in formaldehyde, which makes it stiffer.
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