A forum to address questions regarding mouse models. | Contact experts in Mouse Models to get answers
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Questions related to Mouse Models
I need to know what precautions need to be taken for using CCl4 in the mouse model of inducing liver fibrosis. I plan to inject the CCl4 intraperitonealy. The biosafety comittee has questions as...
14 August 2019 6,812 3 View
In an experiment where you are testing the effects of two drugs on tumor burden, how do you account/normalize for changes to body weight? For example, if one drug results in slower weight gain in...
08 August 2019 7,043 3 View
I found a protocol that uses a single dose heparin in kidney ischemia reperfusion injury induction, I wonder if it`s applicable in the heart. What dosage should I use for cardiac IR model in...
08 August 2019 4,552 1 View
08 August 2019 4,624 2 View
I found different LPS from E. Coli with different serotypes. Is there any difference between O111:B4 serotype and O55:B5? I would appreciate if someone could provide me with a good protocol of...
07 July 2019 8,590 1 View
there are many mouse models that inoculate human cells, such as tumor cells or T cells, to the mice, whether could it induce the phagocytosis of the mouse macrophages to human cells? even in the...
05 May 2019 8,392 3 View
Dear researchers, could anyone please tell me if it is possible to use the Autocal tool available in Mike zero engine to autocalibrate a model built in Mike Urban/ MOUSE? Since Mike zero only...
04 April 2019 5,534 1 View
I did not find any and don't know why.
03 March 2019 3,270 1 View
Multiple research groups have tested petide-human Fc fusion proteins in mouse models, with no apparent adverse effects. However, I can't find any information on the difference between using human...
09 February 2019 8,082 1 View
Does anyone have any experience of how to pharmacologically inhibit Notch signaling in vivo? I am working on mouse models of breast cancer, and would like to block Notch1-mediated signaling in the...
30 January 2019 600 2 View
I have just started using the intraluminal transient MCAO (30min) method in c57BL6 mice. I am thinking of including rotarod testing in my experiments, but have some questions about the protocol....
09 January 2019 2,047 2 View
For The mouse model of oxygen-induced retinopathy, why we put Neonatal mice and their nursing mothers in a 75% oxygen supply chamber from postnatal day 7 (P7) to P12. Why not day 6 or 8 ? what the...
01 January 2019 8,679 0 View
01 January 2019 4,311 3 View
Hi everyone, I am planning a mouse experiment in which I would like to block the FcR in vivo (before I use KO mice). I have been using the 2.4G2 Mab to block the FcR in splenocytes in vitro and...
01 January 2019 3,793 1 View
Basically this method involves spinning tumor cells into a slurry, which is then directly placed onto the tissue. My mouse model is a dorsal skinfold chamber, so the slurry would go directly onto...
12 December 2018 4,775 0 View
Hi Everyone, I am planning a study to test the effectiveness of a cancer therapy on reducing tumor volume in a 4T1 mouse model. In addition, I would like to see the effect of my treatment on the...
12 December 2018 2,435 3 View
What are the precautions that should be taken during immunohistochmestry stating of the articular cartilage of knee?
12 December 2018 1,051 1 View
Toxoplasma gondii strains are remarkably clonal, and virulence in the mouse model is strongly influenced by the genotype of the parasite. Toxoplasma gondii infection in mice after...
12 December 2018 3,421 1 View
10 December 2018 3,362 3 View
Hi, I'm trying to evaluate a paper that looks into IL-1, MMP3 and atherosclerotic plaque stability and would really appreciate advice if anyone has read this paper or knows about the subject ;...
11 November 2018 4,002 0 View
DOES ANYBODY know the choice of mouse model for ovarian cancer with treatment of immune theropy?
10 October 2018 3,647 0 View
Dear all, I would like to know if there is anyone who know a research group or a paper on mouse modeling studying insl3?
10 October 2018 8,822 0 View
The differentiation protocol of mouse CD34+ bone marrow cells into dendritic cells is well known. Is anybody familiar with protocols describing the differentiation of mouse CD34+ BM cells into...
17 September 2018 7,305 3 View
What are some good diet induced mouse models and what are there restrictions?
09 September 2018 1,324 0 View