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Questions related to Mouse Models
I am starting a project to treat cancer by controlling T regulatory cells or by CD4 T cells anti-tumor response. I need to know what tumor type and mouse model I should use ? thank you
09 September 2018 8,968 2 View
I want to isolate the cells from the tumors (mouse model and human). I read that Collagenase I is harsher than Collagenase IV. Can anyone please suggest if there is any commercial kit with the...
09 August 2018 5,066 2 View
08 August 2018 730 2 View
We have an approach that we are evaluating in a mouse model of stroke which stimulates vessel development. We would like to show that vessel development is the key mechanism responsible for the...
08 August 2018 4,146 4 View
We purchased some optrode implants for use with optogenetics and electrophysiology in mouse models. In the past, we have used superglue+dental cement to adhere the implants securely to the skull;...
07 July 2018 1,060 0 View
I am developing a cancer mouse model. I injected a pancreatic cancer cell with two variant WT and KO(some gene) in nude mice subcutaneously. After 4-5 days all mice died in KO batch but all mice...
07 July 2018 6,334 12 View
We would like to setup an orthotopic mouse model of colon cancer, however we are having trouble injecting into the caecal wall (we almost always end up injecting inside the colon). Any...
07 July 2018 3,948 0 View
Hello all, I'd like to get further insights on the disadvantages of using knockout mice/global knockout. Also, how can the one further study the effect of this gene/s' knockout. Thanks in...
07 July 2018 5,411 3 View
Recently I've been studying on ICG related materials' photothermal effect, which need a 808nm diode laser system. Are there any recommendations of reliable laser supplier? What's more, from other...
06 June 2018 5,077 3 View
Hi, guys, I did DSS-induced colitis model recently to find whether my targeted herb has the anti-inflammatory or immunomodulatory function in inflammation mouse model. However, I find the ratio of...
06 June 2018 1,976 0 View
When using MUGA genotype data for QTL analysis: What is the best way to determine the strain specific genotypes from SNPs with JAX or UNC ids rather than an rs id? Examples: JAX00288313, UNC120396195
06 June 2018 4,273 0 View
Interested in developing a skeletal-muscle tissue specific genetic knockout in a mouse model. Any relevant methods papers or resources would be appreciated!
05 May 2018 8,275 3 View
Dear all, I am trying to develop EAE mouse with MOG peptide & Mycobacterium tuberculosis as an adjuvant & PTX. But I could succeed over it. I think I could not able to administrate the...
05 May 2018 7,249 2 View
Hi! I work with a mouse model that expresses the Cre recombinase, so that only upon tamoxifen stimulation the animals express Cre, thus I can induce the KO of the floxed gene. I know that in vitro...
04 April 2018 1,392 4 View
We are interested in accelerating the functional recovery following a mechanical insult to sciatic nerve in mouse model. A detail mechanism of injury type, molecular and morphological changes, and...
04 April 2018 7,028 0 View
Anyone can suggest a mouse model of Alzheime's disease with specific dysfunction in the basal forebrain cholinergic system?
03 March 2018 9,660 2 View
I have to perform RNA-seq for a muscular dystrophy mouse model. I would like to get suggestion from the researcher who have worked with/ done RNA-seq using skeletal muscle derived RNA.
03 March 2018 526 0 View
I would like to know the recommended dose of teicoplanin IP injection for a BALB/c model in mg/kg, and whether it should be divided or not. (for treatment of a bacterial infection in combination)
03 March 2018 7,915 2 View
Looking for a mouse model for inflammatory colitis, specifically through TNF modifications.
03 March 2018 4,705 0 View
I am working on a mouse model of B cell lymphoma. I would like to use dexamethasone as an anti-inflammatory drug to combine with chemotherapy in my model, as suggested in Appelmann et al, Blood...
03 March 2018 9,887 0 View
Hi all. Could you please help me on how to choose a female for a sexual preference experiment in mice? I have read that it should be a receptive, in estrous mouse, but I am not sure how to...
03 March 2018 419 6 View
Currently, there are many methods or algorithms to predict neoantigen in human samples. However, if we would like to predict neoantigen from murine cancer cell lines in C57BL6/J background, does...
22 February 2018 778 1 View
I am working on a mouse model with fatty liver and am trying to extract IHL by ficoll protocol and I can't get good yield of lymphocytes. The issue is that I always get a layer of fats at the...
02 February 2018 8,232 6 View
I had experimental result in EAE induced mouse model :Spleen total cell decreased :Acute EAE Phase(clinical score about 3), Recovery Phase( clinical score about 2) I sacrificed mouse(n=3) weekly...
01 January 2018 1,949 1 View