I am Working on Primary Neural Cell culture from SVZ of the Postnatal Mice Can anybody Help me to Provide Some guidance how to Precisely cut this part. and How to recognize SVZ in Brain of Postnatal mice. If you have some Videos Illustrative Diagram I could be better for me?
Xiwei Shan
Hi Masood,
I wonder if you have seen this JOVE article?
This is a protocol for whole-mount immunostaining but you can follow it's steps of dissection. It is mostly applicable to all postnatal brains. For new born (p0-5) the brain shapes slightly differently, but the general gist is there.
Let me know if you need more info!
Xiwei
Article The Subventricular Zone En-face: Wholemount Staining and Epe...
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