What is the best way to reconstitute and administer nitrofen to mice?

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Ossama Mohamed Zakaria

Please read the following references:

1- Keijzer R, Liu J, Deimling J, Tibboel D, Martin Post M. Dual-Hit Hypothesis Explains Pulmonary Hypoplasia in the Nitrofen Model of Congenital Diaphragmatic Hernia.Am J Pathol. 2000 ; 156(4): 1299–1306.

2- Wu CS, Chen CM, Chou HC.Pulmonary Hypoplasia Induced by Oligohydramnios: Findings from Animal Models and a Population-Based Study.Pediatr Neonatol. 2016 May 29. pii: S1875-9572(16)30062-6.

Mathangi Chandramouli

Thank you both for your answers!

I was actually looking for how to prepare the nitrofen before administration, as it is not known to be soluble in anything (poorly soluble in olive oil). Also, I was trying to see if there are any suggestions as to the best way to gavage these mice, as we have run into some gangrenous bowel in the past.

Thank you!