Does any one know about HES staining??

Actully I want to use Hemtoxylene eosin and safran (HES) staining for my bone tissue sections , can anybody guide me regarding its protocol??

03 April 2019 10,024 1 View

Can any one guide me in explainng this picture??

HI everyone, I just isolated my RNA from directly from blood by TRIZOL method, I got purity of 1.97 (260/280) which is pretty in acceptable region, but when I run my RNA on gel it shows 3 bands....

31 December 2018 7,760 8 View

What should be the dissociation curve in real time PCR if my primer has similarity with its own transcript variance?

If my primer has the specificty with transcript variance of its own, then what should be the the possiblity dissociation curve of my real time pcr and how its effects my results?

01 February 2018 7,272 4 View

Protocol for 20s proteosomal activity assay??

what is the protocol or method to check the ubiquitin proteosomal in activity in brain samples as i also have flourogenic AMC as substrate for proteosome.

11 December 2017 9,596 0 View

Can anyone tell me how to calculate dilution factor ?

08 September 2017 3,174 5 View

Primer designing

can anyone tell me what would be the change in the bands on gel if i use primers from exon-exon junctions and non exon-exon junction??

07 August 2017 7,628 5 View

Grip strength test in rodents??

I have an older version of grip strength meter(GSM) UGo basile cat # 47105-001 can any one tell me how i can use it?? Actually its user mannual have lost and i cann't find user mannual of this on...

02 March 2017 7,321 0 View

Bouine's fixative??

Can any one tell me how to prepare bouine's fixative in lab form powder picric acid and 37% formaldehyde???

02 March 2017 3,682 1 View

What is the effect on PCR results if two PRIMERS have different GC %??

Read 4 answers by scientists with 4 recommendations from their colleagues to the question asked by Zaid abdul razzak on Oct 24, 2016

09 October 2016 9,394 4 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View