Hi Jasmien,

Fortunately, I still keep on the back of my mind a patent published in 2012, which exactly fits your request, as it is also related to the maximum tolerated dose of doxorubicin. Namely it US 20120100218 A1 Patent entitled "Intralymphatic Chemotherapy Drug Carriers". As it follows from patent' abstract, a chemotherapeutic composition can be configured for subcutaneous administration for preferential intralymphatic accumulation while also providing a therapeutic systemic concentration that is not toxic. The composition can include a pharmaceutically acceptable carrier, and a nanoconjugate configured for preferential intralymphatic accumulation after subcutaneous administration. The nanoconjugate can include a nanocarrier configured for preferential intralymphatic accumulation after subcutaneous or interstitial administration, and a plurality of chemotherapeutic agents coupled to the nanocarrier. The nanoconjugate can have a dimension of about 10 nm to about 5 nm. Also, the nanoconjugate can be loaded with the chemotherapeutic agents from about 10% to about 50% w/w. The nanocarrier can be a hyaluronan polymer of about 3 kDa to about 50 kDa. Alternatively, the nanocarrier can be a dendrimer.

Best wishes,

Ilya

Hi Jasmein,

Preferably, The dose of  Dox (2 mg/kg), intraperitoneally inject into the nude mice once in a week. The suggestion providing to dissolve the dox in saline soluition.

Remarkably, multiple cytotoxic mechanisms are involved in the pathogenesis of Dox-induced toxicity. However, a large body of evidence indicates that Dox-induced oxidative stress remains the cornerstone, as evidence by reactive oxygen species (ROS) induced oxidative damage such as lipid peroxidation.

Best Regards,

Mohamed Thoufic,

Hi Jasmien,

Since IP injection of Doxo has very toxic local effect for intestine, pain etc. our collaborators performed pilot study to optimize Doxo dose in C57BL mice for inducing cardiotoxiticity. The best results obtained from tail vein injection. No animal loss registered for Doxo dose of 4 mg/kg and 20 mg/kg at endpoint (9 days) after injection in both sexes.

The most important things to control water availability since animal very weak after injection and sometimes not able to use central water supply system or bottles. So put transportation gel or just food pellets soaked with water available for animals.

It should help a lot.

Daniil