I'm looking for a good protocol for sectioning frozen undecalcified rat bone. My peptide of interest is very small and vulnerable to degradation (it will probably not survive decalcification or processing for paraffin embedding). I am able to embed bone in OTC and section using a disposable blade however the sections are very ragged. I've heard about the Kawamoto tape method and the cryoJane method, but I was hoping there was a less expensive solution.
Any advice would be greatly appreciated. Thank you.
Dear Michelle,
It may be helpful to start from young age rats. I used to work on cochlea and had similar issues with the bone. Up to around postnatal day 14 you should be able get pretty good sections without decalcification, I guess that depends on which bone you are cutting.
best wishes, Refik
speicmen were immediately fixed in 10% formal saline followed by treatment with 2% potassium hydroxide for 5 days till ossified areas were clearly visible through the soft tissue.
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