What is the meaning of pale stained nuclei with hematoxylin?

I am staining pancreas with HE. I found beta cells show pale hematoxylin staining in conditional knockout mice compared to surrounding other islet cells, exocrine and acinar tissue " this means...

03 April 2018 234 1 View

Normalization by DNA content in glucose stimulated insulin secretion assay for islets?

I want to ask about normalization step: if I want to normalize by DNA content how I should isolate DNA after treatment of the islets with acidified ethanol?

07 August 2017 4,407 0 View

Has anybody faced difficulties with islets isolation from db/db mouse model?

Recently, I am trying to isolate islets from db/db mice. I use the same protocol I regularly use for islets isolation from mice but I can't get any islets or very few pale islets attached to...

01 February 2017 5,872 7 View

For murine islets, can I work on islets extracted from one mouse or I have to pool from several mice?

I am recently extracting murine islets. I see most researchers recommend pooling islets from several mice. I just want to check expression of my protein of interest in islets as an initial stage...

10 November 2016 9,330 3 View

What is the best internal control for western blots of mice lysates?

09 October 2016 6,228 3 View

I am asking if somebody previously transfected islets of Langerhans isolated from mice with siRNA before. Is this going to work?

If this works what kind of tranfection reagent is recommended for the islets?

07 August 2016 4,448 9 View

Are beta actin and tubulin levels in islets affected by fasting ?

I am interested in knowing the expression of a certain protein in the islets under feeding and fasting conditions. I use the same total protein concentration for immunolotting. In my first trial I...

07 August 2016 4,264 1 View

I want to ask about measurement of insulin content in MIN6 cells?

I did a glucose stimulated insulin secretion assay for MIN6 cells. I measured the released insulin in the Kreb's Ringer buffer containing certain concentrations of glucose. In the end I collected...

06 July 2016 3,844 2 View

Which transfection reagent is best with reporter assays on MIN6 cells?

I want to transfect PGL3 plasmid to MIN6 cells. I tried Trans-it trnsfection reagent but didn't work well.

01 January 1970 5,237 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View