Hi everyone,
I hope this email finds you well. I have two questions about breast tumor xenografts, which I hope to find answers from you.
1. To assess metastasis from tumor xenografts, what marker(s) have you looked for? Also, what technique(s) do you employ, for example, histology or western blotting?
2. I will be mixing MCF-7 cells with matrigel before injecting the cells into the mammary fat pad of my nude mice. Eventually, I will cut out the tumors to weight them and for molecular analyses. For this process, how do I make sure that I will only cut out tumor tissue and not healthy tissue?
Sincerely,
John Tat