I know this type of assay is better done with a rat however our circumstances left us using a mouse model. That being said, its pretty difficult to get this aortic ring to respond to much of anything.
Our setup:
Our procedure:
Our responses thus far have been pretty much nonexistent. Any advice or tips would be appreciated. I am attaching an image of a recent test where we think we got some sort of response, just not anywhere near where we thought we would be (for instance the highest change in tension in this experiment was ~ 70mg whereas we've read people have as high as 400mg).
Thanks!