Can someone give me the protocol to study mitochondrial Calcium overload, in adult cardiomyocytes?

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At what density i should seed my cardiomyocytes (isolated from neonatal mice) in a 35mm culture dish?

at what density i should seed cardiomyocytes (isolated from neonatal mice) in a 35mm culture dish? and what should be the final volume (0.5, 1 or 2ml )? i have to use this for ROS detection using...

03 April 2018 5,139 2 View

What could be used as a positive control for reduced infarction size (myocardial ischemia)?

which drug/reagent would be the best positive control to compare and determine the effect of a plant extract (with strong antioxidant properties) on reduced infarction size (myocardial...

10 November 2017 627 4 View

SiRNA used is overexpressing the lncRNA, WHAT COULD BE THE PROBLEM?

i am trying to knockdown a lncRNA using siRNA. with lipo2000 and Opti-mem. ..This experiment has been repeated 3-4 times in lab by different people. but now when i tried to do knockdown,, lncRNA...

05 June 2017 9,894 7 View

Which cell line is best to study atrial fibrillation (specially remodeling)?

which cell line would best to study atrial fibrillation (OR lncRNA role in atrial fibrillation) . and if we have to use mice/ rat model for invivo study, then which strain would be best? AND WHY...

03 April 2017 7,807 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

What are the options for global scale hydrology DEM?

Hello, I am working on a global scale hydrology GIS project delineating global watersheds and doing some data analysis. I have in the past used hydro1K from the USGS that cover the entire world....

18 February 2021 5,109 5 View

Can I use a categorical covariate in a Generalized Linear Model in SPSS v.26?

Hello everyone, hope you are well. I am planning to use a Generalized Linear Model in SPSS. I would like to add gender to the model as a covariate, however from what I have read, only continuous...

14 February 2021 8,937 4 View

Mixed ANOVA or Multilevel modeling?

Hi everyone, I'm having some questions about choosing the right test for my experiment and I hope I can find help here! Here are the settings of the experiment: 1. participants are randomly...

14 February 2021 1,028 4 View