Can someone give me the protocol to study mitochondrial Calcium overload, in adult cardiomyocytes?

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At what density i should seed my cardiomyocytes (isolated from neonatal mice) in a 35mm culture dish?

at what density i should seed cardiomyocytes (isolated from neonatal mice) in a 35mm culture dish? and what should be the final volume (0.5, 1 or 2ml )? i have to use this for ROS detection using...

03 April 2018 5,216 2 View

What could be used as a positive control for reduced infarction size (myocardial ischemia)?

which drug/reagent would be the best positive control to compare and determine the effect of a plant extract (with strong antioxidant properties) on reduced infarction size (myocardial...

10 November 2017 702 4 View

SiRNA used is overexpressing the lncRNA, WHAT COULD BE THE PROBLEM?

i am trying to knockdown a lncRNA using siRNA. with lipo2000 and Opti-mem. ..This experiment has been repeated 3-4 times in lab by different people. but now when i tried to do knockdown,, lncRNA...

05 June 2017 9,967 7 View

Which cell line is best to study atrial fibrillation (specially remodeling)?

which cell line would best to study atrial fibrillation (OR lncRNA role in atrial fibrillation) . and if we have to use mice/ rat model for invivo study, then which strain would be best? AND WHY...

03 April 2017 7,869 3 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

Why can't academics earn the money they deserve?

Only Journals make money from the articles we have worked on for years. Academics do not earn money from their refereeing. Then shouldn't the solution be a system in which academics can earn...

01 August 2024 6,469 6 View

Posthoc test lettering in JAMOVI?

Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...

31 July 2024 3,333 4 View

Where to download DEM from 2018?

I am currently working on validating land subsidence results obtained using SBAS InSAR. Could anyone suggest reliable sources or repositories where I can download a historical DEM from 2018?...

31 July 2024 9,757 3 View

SAS Generalized Linear Model for trial/event anaysis and not survival (time to event) analysis?

I am looking for a published article using SAS or SPSS Generalized linear model for trial/event data and not survival analysis. Both software packages off the option for the number of success out...

30 July 2024 3,835 2 View

What are the perfect mathematical model for a solar-powered ship implementation?

A computational study of heat flux on cylindrical surfaces using CNT hybrid nanofluids

28 July 2024 4,985 1 View