What are the parameters for selecting a transcription factor bound to promoter region when using PROMO:Alggen?

I am interested in finding transcription factors binding to promoter of a virus, Merkel cell polyomavirus. I got several factors with varying dissimilarity. Is there any criteria that can be used...

04 May 2016 632 2 View

How do I find promoter sequence of a viral genome ?

I want to align promoter sequence of a small T antigen and large T antigen of virus (merkel cell polyomavirus). I am aware that for human genome we generally subtract and add 1 kb from genome to...

09 October 2015 8,022 1 View

How to choose a journal to publish your paper?

I have some data from my MSc dissertation which I am thinking of publishing but I am not sure how to publish a paper eg how to choose a journal, impact factor etc. Can anyone provide...

01 February 2015 484 6 View

How much of Bioinformatics knowledge do I need to elucidate the functional effect of missense mutation?

I am a postgraduate student and have completed MSc in Medical genetics. I intend to apply for PhD in future and my current interest lies in analysing missense mutations for their pathogenecity. I...

31 December 2014 7,138 3 View

Does anyone have experience in generating workflow in CLC Genomics Workbench 7.5?

Hello everyone, I am supposed to use CLC Genomics workbench to perform trio analysis to find candidate gene responsible for rare disorder. Can anyone help me in generating Workflow in CLC Genomics?

09 October 2014 8,053 2 View

How can one perform trio analysis using CLC genomics workbench 7.5?

I have a exome sequencing data of parents and their child. We are trying to identify the candidate genes responsible for some abnormality.I have never used CLC genomics workbench. Do I need a...

09 October 2014 3,310 4 View

Computing h bonds in swiss pdb viewer?

I wanted to see the h bond interaction within a protein so I used swiss pdb viewer. Generally it gives only green dotted line as h bonds, but it also gave purple colored dotted line. Can anyone...

07 August 2014 1,714 1 View

Can anyone explain me the russell linding score?

07 August 2014 7,552 2 View

Is there any criteria to choose a chaperon to crystallize a intrinsically disordered region in a protein?

I read an article where an intrinsically flexible protein was crystallized using chaperon (here chaperon is a protein that helps to stabilize other protein) . i was wondering what would be the...

07 August 2014 7,548 5 View

For using Align GVGD we have to paste MSAs, so how does one choose the number of sequences that share a similarity between the gene of interest?

I am trying to analyse APC gene. I used blast to get multiple sequence alignment. How many sequences should I choose to input in Align GVGD. Is there any criteria?

06 July 2014 6,001 1 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View