I'm currently working on a mouse model that becomes obese and glucose intolerant at the age of 7 months when fed standard chow. Since I'm primarily a reproductive biologist, I have a naive question: do I need to do a high fat diet experiment? My hypothesis is that then the mice might just become more obese and maybe at a younger age, but would this experiment be necessary? What crucial information could I gain by doing this experiment?
The experiment will actually tell you whether your model becomes more obese and glucose intolerant than a normal mouse in response to a High fat challenge. People do it because it is faster than the "aging" experiment you have done and in some cases it is required to reveal the phenotype. You do not need to do this experiment but it will be a nice addition to your observations.
Obesity in mice can arise from either monogenic disorders or from complex interactions between genetic background, maternal-fetal environment, learned behaviors and external environmental variables such as diet composition, ambient temperature, threats from predators, and so on.
High-Fat Diet Formula:
HFD that consists of 58% fat, 25% protein and 17% carbohydrate, lard (13%), cholesterol (1%), vitamin, and minerals (0.6%) as a percentage of total kcal ad libitum, respectively, was administered every (5 ).Food intake was calculated every day and bodyweight was measured once in every two days.
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