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28 March 2019 5,253 2 View
I have schiff base proligand and I need to use for making schiff base metal complex. I have difficulty to dissolve this schiff base proligand. It is unsoluble in Ethanol and Methanol even after...
25 March 2019 6,519 4 View
i would life for it to have no effect because when it was only ethanol extract it inhibited pseudomonas aeruginosa.
14 March 2019 2,529 2 View
In bioassay studies, the effect of essential oils is used as an insecticide from acetone, but is used to study the microbial activity of essential oils from DMSO solvents. Why?
13 March 2019 5,849 7 View
I am working on synthesis of drug loaded microspheres by using resistant starch as a coating material, my polymer is only soluble in DMSO, and it is nonvolatile in nature how could I possibly...
05 March 2019 7,561 2 View
Does anyone know how long an aqueous solution of SCH 50911 ((2S)-(+)-5,5-Dimethyl-2-morpholineacetic acid), a GABAB receptor selective antagonist, can be stored and at what temperature? In...
21 February 2019 4,980 1 View
Mark–Houwink–Kuhn–Sakurada coefficients for finding the molecular weight of amylopectin from cassava starch using 90% DMSO is available in any literature?
16 February 2019 4,310 1 View
Dear All, I'm now trying to amplify a gene with 3690bp from the genomic DNA. I've design 3 sets of primers. First set is flanking the whole gene. Second set is flanking from 1 to 2613bp. Final...
08 February 2019 1,337 1 View
I am experiencing some toxicity troubles during the treatment. Basically, my zebrafish did not survive after 2hs of treatment. I am using 5 microM TAM from a stock dissolved in 100ml conditioned...
31 January 2019 3,543 1 View
Hello everyone, Currently we are trying to do some pharmacological studies with thalidomide in vitro as well as in mice models. Regarding in vitro, does someone have expertise using it? I am...
16 January 2019 2,415 3 View
How long can CCCP (10mM) be stored in DMSO for at -20 celsius?
07 January 2019 4,989 3 View
Dear all, I am planning to use for in vitro experiments Volasertib (BI6727) that I obtained from Cayman. I obtained 1mg power that I am planning to dissolve in DMSO. I was wondering whether...
05 January 2019 8,024 2 View
I synthesized a zinc oxide nano-particle i have checked but it could not dissolved in DMSO. i want to know the genuine reason and some authentic solution.
05 December 2018 9,853 4 View
We are currently working on a photopolymerized hydrogel using riboflavin as a photoinitiator. The riboflavin we have is not soluble in water and needs DMSO to make it soluble. There are concerns...
04 December 2018 5,183 1 View
Is it appropriate to use DMSO:Corn oil mixture (50:50) in mouse as IP? I heard corn oil can induce colitis as IP, but there are many researchers using corn oil as in vivo test. I wonder your...
04 December 2018 4,262 1 View
I am currently aiming to proliferate HUVEC cell line in vitro. What would you suggest as an optimum freezing media(whether with or without EGF), FBS, and DMSO ratio?
29 November 2018 6,437 4 View
To characterize my synthesized block copolymer PAA -b-PNIPAM (PAA=Polyacrylic Acid), I need to take the NMR test. DMSO was the only solvent I found on literature. But I couldn’t get it dissolved...
29 November 2018 4,245 2 View
Hello All, I'm working with MDA-MB-231 cell line and I always perform same procedure while I'm passaging these cells. After unfreeze the cells ( at first I freeze them with 5% DMSO, 95% FBS...
22 November 2018 4,915 12 View
I'm running a conjugation reaction to label 20nt oligonucleotides with an NHS ester fluorophore. The oligos have a 3' amino group to anchor the ester. I dissolved the NHS ester in 100% DMSO and...
13 November 2018 4,074 3 View
Hello there, I'd like to ask whether there are any cryoprotectants/cryopreservants medium with no DMSO in them. Specifically to use it for freezing tissue such as collagen scaffold. I have found...
13 November 2018 9,106 3 View
The cells are cryopreserved in 8% DMSO and 2% HSA.
09 November 2018 3,983 3 View
We have several essential oils we want to test in cell culture for cell viability using XTT assay. We only have 500 ul or less of each oil. We want to test these oils at concentrations that range...
25 October 2018 4,166 2 View
I have been doing MTT assay for testing cytotoxicity of thio-containing compound. After adding 50 microlitter/well, I put the 96-well plate back to incubator within 4 hours. Instead of appearing...
25 October 2018 4,113 5 View
I am using the same kinase inhibitor and in in vivo experiments after dilution with the permitted conce, of DMSO less than 10% I saw the white percipitation in injection rout (IP). Also I...
21 October 2018 9,193 2 View