Dear Mahmoudvand,

Dimethyl sulfoxide with molecular weight 78.13 g/mol, attributed with a wide range of bioactivities including analgesic, anti-inflammatory, and cryoprotective properties. It's a very hygroscopic liquid and practically no odor or color but slightly bitter with a sweet after-taste. DMSO rapidly absorbed through the skin and mucous membranes; enhances dermal absorption of many other chemicals. It is solvent for many organic compounds including fats, carbohydrates, and polymers in organic reactions. Itself is toxic for pest and I think is not suitable for pesticide bioassay. 

However, acetone with boiling point 56.5 degrees Celcius is a common solvent for your purpose. It is a prevalent solvent for fats, oils, waxes, resins, and plastics. It has middle solubility in both water and oils. Therefore, acetone is the best choice for bioassay. 

Sincerely yours, Homayoon.

After reviewing some of the articles that you have published, I observe that to measure the repellent activity of certain essential oils against selected insects, you use as a standard methodology the application of solutions of these oils in filter paper discs. In this type of test, as only the repellent effect of the essential oil is of interest, the solvent is allowed to evaporate for a period of 10 min. The convenience of using acetone in these tests lies in its volatility, the boiling temperature of acetone is 59 ° C. DMSO is much less volatile since its boiling tempearture is 189 ºC. If you use it, you should let it evaporate for a much longer time. At least in this type of test the use of acetone is a matter of practicality, that is, if acetone is as good solvent as the DMSO of the essential oils that you examine, acetone is preferred because it is easier to remove from the discs .

The use of DMSO in antibacterial tests is due to other reasons. This solvent is more versatile; because it is not as polar as acetone, it is more appropriate to dissolve a greater number of substances of low polarity or high lipophilicity, such as essential oils. Although it is generally not possible to remove DMSO from the culture medium in which the bacteria are found, the added amount of this solvent in both treatments and controls is adjusted to levels that do not produce significant cellular toxicity.

Hi,

DMSO is toxic itself against insects and sometimes it may innteract with your essential oil. As far as I know, acetone is more easy to use and widespread in scientific papers.

Best wishes,

AZ

Hi, Dear Duque-River

Thanks of answering

Best Regards

S.Mahmoudvand

Salam Dr Zibaee aziz

Motshakeram az pasokh arzeshmand shoma.

Ba Ehteram

S.Mahmoudvand

Dear Mahmoudvand,

Dimethyl sulfoxide with molecular weight 78.13 g/mol, attributed with a wide range of bioactivities including analgesic, anti-inflammatory, and cryoprotective properties. It's a very hygroscopic liquid and practically no odor or color but slightly bitter with a sweet after-taste. DMSO rapidly absorbed through the skin and mucous membranes; enhances dermal absorption of many other chemicals. It is solvent for many organic compounds including fats, carbohydrates, and polymers in organic reactions. Itself is toxic for pest and I think is not suitable for pesticide bioassay. 

However, acetone with boiling point 56.5 degrees Celcius is a common solvent for your purpose. It is a prevalent solvent for fats, oils, waxes, resins, and plastics. It has middle solubility in both water and oils. Therefore, acetone is the best choice for bioassay. 

Sincerely yours, Homayoon.

Salam Mohamad Homayoonzadeh

Sepas of pasokheton.

Ba Ehteram

S.Mahmoudvand