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Questions related to DMSO
I have prepared sulfonated poly ether ether ketone for the polymer electrolyte membrane but it is not going to soluble in solvents like DMSO, DMF, DMAc.Please guide me where the problem is?
15 October 2020 7,768 1 View
Dear all. I am about to start B cell immortalization experiments with EBV, but the vast majority of my blood samples come from frozen whole blood in EDTA tubes and without any cryopreservation...
08 October 2020 7,225 2 View
The product came as a powder and needs to be stored at -20 degrees. It can be diluted in DMSO but I don't know if I should store it still at -20 or other condition.
05 October 2020 8,718 2 View
I noticed that drugs or reagents dissolved in DMSO and stored at -20C become liquid after a year or two of storage. Does anyone know why this is and if it affects the effectiveness of the...
01 October 2020 8,162 2 View
I would be very grateful for any help regarding my problem. My research requires applying betulin (and betulinic acid) solution on the cerebral cortex slices of rats. With the stock solution of...
28 September 2020 9,025 2 View
I have 2 drugs (PTC-209 and GSK343) that are soluble in DMSO (I already made the stock solution, dissolved to a clear solution) and I have to inject them IP to mice for a 2 week treatment course....
18 September 2020 9,504 3 View
I have the evidances of radical involvement in a reaction where DMSO is acting as oxidant. I want to find a reported example.
18 September 2020 2,159 1 View
Hi all, I have problem with my hepG2 cells. So before covid lockdown, I froze my cells and put it in Mr Frosty in -80°C. I used Cryostor as the freezing media for 1st batch after I received the...
05 September 2020 6,937 5 View
Hi, I tried several times to freeze H9c2 cells in liquid nitrogen, but as soon as I defrosted them ~6 months later, most of them were dead and I had to expand the cultur from few cells on. This...
04 September 2020 2,554 2 View
Hello, I am about to start working with Cinnamaldehyde and reading the literature about that. I will dissolve it in DMSO like everyone did but I am not sure how to store. Some store it in -20...
31 August 2020 5,885 1 View
Hi everyone, I was wondering if anyone has a solution for the amplification of long GC-regions using PCR? I want to isolate ~5kb long sequence from mouse genome that is unfortunately rich in...
25 August 2020 4,234 3 View
I have a compound stock diluted in DMSO. What would be a good way to give it to mice by oral gavage? What concentration of DMSO could be tolerable for mice if administered by oral gavage ? Thank you!
12 August 2020 8,872 5 View
Hello Dear scientists, is that possible to use DMSO instead of MTT or TTC to evaluate the antimicrobial activity of Bacteriocin in broth?
10 August 2020 4,129 2 View
I have prepared quantum dots using the solvents DMSO and DMF and now I need to dry out these solvents completely without damaging the structure of the quantum dots. Kindly suggest some methods.
07 August 2020 5,216 1 View
I am looking for an organic solvent for trehalose. DMSO works, methanol works, like ethanol, but both are not suited for the rest of my process. Any ideas or exprience here?
05 August 2020 7,199 3 View
So I need to store these for 4 weeks at least, before I do a Dyna bead negative T cell isolation on it. What should I store it in FBS? or DMSO and at 4 degrees? or is -20 not good for the cells?
27 July 2020 2,728 3 View
Dear scientist, what final concentration of DMSO should be used for 3T3-L1 cells? Some people say 0.1% while some reported 0.5 %? Will it be much more toxic if I use 0.5 % DMSO as a vehicle for my...
16 July 2020 8,856 2 View
I am working with platinum(IV) prodrugs. Most of the synthetic procedures states that after most of DMSO is removed the residue should be treated with methanol and ether, giving the desired...
13 July 2020 1,816 3 View
How these solvents limit shrinking ice crystals during freeze-drying or vitrification? and what are the main differences between DMSO and Glycerol? where each one is useful? I will be grateful if...
18 May 2020 3,832 1 View
Often, any ferrocene derivative during its characterization using a spectroscopic method gives two absorbances, along the ultraviolet region and the visible region (figure 1 ). in vitro was...
06 May 2020 4,799 6 View
Hi I have a 4+ charged aromatic macrocyclic dimer which is soluble in DMSO, ACN and other polar solvents. I have interesting phenomena in DMSO-d6 and ACN-d3. In DMSO d6, my aromatic proton...
12 March 2020 9,803 1 View
We need a stock solution of 5 μM laurdan in DMSO. After prolonged vortex, laurdan did not completely dissolve in DMSO. Someone knows how to improve the process of solubilization of laurdan in...
09 March 2020 9,437 3 View
Due to the high b.p of DMSO and DMF (189 and 153 degree Celsius), it is difficult to recover my polymer from the solution. I believe extraction in a triple system (such as DCM, DMSO and water)...
05 March 2020 3,406 12 View
My hydrophobic peptide (12 AA) is soluble in 100% DMSO. Given that I should encapsulate it in hyaluronic acid nanoparticles, I need that peptide is soluble or partly soluble in water. Does someone...
04 March 2020 8,275 4 View