How to exclude direct effect of drugs to neurons in mouse parkinson's model?

26 October 2020 1,127 2 View

LC3I band detection?

Hi, I have some questions about the electrophoresis and transfer condition. In our lab, we used 15% gel to SDS-page and semi-dry transfer In electrophoresis, i migrated the stacking gel in 80 V...

14 October 2020 4,046 2 View

How can i solve this problem, OPV active film getting hazy?

Hi, guys.. These days, I have some problem in OPV processing. To be more specific, the active film is OK right after spin casting. But while drying it, the film is getting hazy..... it is changed...

07 January 2020 7,673 4 View

How to make SnO2 thin film by SnCl2-2H2O?

26 November 2019 5,407 3 View

What size of gold nanoparticles should I synthesize?

13 July 2019 5,878 2 View

how high temperature pepper fruits can resist?

I hope to monitor how fungal conidia (colletotrichum acutatum) infect on dead pepper fruits. so, I dipped sliced fragments of a pepper fruit in boiling water for 30 seconds and inoculated fungal...

11 July 2019 6,618 3 View

Do you know reason for wrong output on HOMO and LUMO energies in Gamess?

In the output result, I found that HOMO and LUMO energies were too high. I wonder what is the problem in here. Please check attached files. In first file, NPREO was used to print homo-1, homo,...

23 August 2018 7,738 7 View

Has anybody tried the cellulose-based DNA clean-up method?

Hello colleagues, Nucleic acid purification from plants, animals and microbes ... I wonder if this method works in other people's hands, particularly for adherent mammalian cells. I also wonder...

04 May 2018 3,729 0 View

The usage of clock circuit in micro controller chip?

Why do we use clock circuit when we are designing certain micro controller onto PCB? What it is usage? What other circuits do we need in order to proceed? Would you be able to give some advice...

03 January 2018 8,975 12 View

When is the timing changing the cells ?

We use cells as doing subculture (ex: cancer cells). However I don't know of the timing changing the cells. When is the timing changing the cell ?

26 October 2017 4,525 6 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

How can Gamma Ray API units be converted to CPS (counts per second)?

Oil and gas companies run gamma ray tool and take readings in API units while Gamma ray is also noted in cps, so how these units can be converted into each other?

24 February 2021 7,545 2 View

How to remove compound ( Europium complex ) from paraffin oil?

How can I remove my compound from paraffin oil? Mistakenly my compound fell in an oil. Compound = Europium complex

22 February 2021 5,667 2 View

When I mix oil and NaOH solution, Why solid layer in the surface is formed?

During my trial for lubricant oil re-refinery process, I use NaOH as coagulant agent. After overnight of gravity settling, a solid layer in the surface was form. what product is that? since the...

22 February 2021 2,116 4 View