In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short... | Contact experts in PCR to get answers
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Questions related to PCR
Is there any way to solve resistance of herbicide?
07 June 2024 8,446 1 View
I have to find the randomness of encrypted image using NIST SP800-22 with(sequence 100 with each 1000000 bits or any number) but there are no such sufficient number bits then when running the...
06 June 2024 9,557 0 View
Hi, I need your opinion on the best medium for cultivating Phytophthora erythroseptica, the fungus responsible for pink rot in potatoes. Additionally, I would like to know how to achieve maximum...
05 June 2024 8,813 3 View
i have a species trichoderma harzianum. It is development in PDA media normally. But it is producing yellow pigment on rice broken at fermentation.
01 June 2024 9,206 0 View
at the preparation of the C18 alkyl silica, the paper used n-pentanol to dissolve PVP, in my lab I have 1- butanol. Could it be the same?
21 May 2024 5,863 1 View
In a semicontinuous cultivation strategy, how to calculate the dilution rate? Is the dilution rate is same as the renewal rate? What are the parameters required during this cultivation?
18 May 2024 4,235 0 View
This forthcoming study aims to gauge public awareness of Precision Medicine and explore how AI and ML technologies can enhance healthcare outcomes. Please consider contributing to this research by...
13 May 2024 7,506 0 View
Hello, Please is goat genome not listed on UCSC In-Silico PCR website? Please could someone assist on what to do if working with goat Thank you
02 May 2024 3,451 3 View
I am using Purelink RNA mini kit (Fischer Scientific) to extract total RNA from bacteria. i need to get better RNA integrity number like more than 7, but for only one sample I got 7.4, for other...
02 May 2024 4,294 3 View
I am doing a research on pharmacogenetics of treatment of type2 diabetes. the SNPs i have selected are in close proximity on the same chromosome. I want to carry out linkage disequilibrium and...
29 April 2024 6,394 1 View
There is no information present in the databases regarding the synthesis of lanosterol nanoparticles.
29 April 2024 2,034 3 View
Dear Researchers What are the initial characterisations utilised to confirm the synthesis of MOF. Kindly suggest. Thank You
25 April 2024 1,359 2 View
Why does the number of exons of PTPRQ change by zooming out in Genome Browser site? the NM number is: NM_001145026.2
22 April 2024 8,277 1 View
For the estimation of beta-carotene by acetone extraction method using petroleum ether and sodium sulphate at 453 nm. it is known to all that the extinction coefficient of beta-carotene at 453 nm...
17 April 2024 3,670 1 View
Since some gene prioritization tools were published in the last decade, there has been no new research in the area of gene prioritization or candidate gene discovery. Can somebody shed light on...
06 April 2024 4,563 1 View
Forecasting using ANN for a single variable. Say, inflation.
04 April 2024 6,477 4 View
I noticed that a lot of plasmid annotations tend to find ColE1 origins, but I can never usually find the b. subtilis origin of replications from annotations.
22 March 2024 7,158 2 View
Hi, I did FTIR of my oxidized biochar samples and found that peak area is larger after deloading of phosphorus rather than loading of phosphorus. So far i know peak area will be smaller after...
16 March 2024 5,048 1 View
Our lab buys 500 mL bottles of RPMI-1640 Medium and stores them at 4C. Oftentimes, for my experiments, I only need about 100 mL total. So, I will make RPMI-1640 + 10% FBS with 90 mL of media and...
23 February 2024 4,472 2 View
I encountered the fluorescence enhancement in the DNA interaction process. Since the classical Stern-Volmer equation is used for the fluorescence quenching process, this equation cannot be used to...
17 February 2024 2,719 1 View
I have faced this problem with different types of tissues whenever i immerse them in formaldehyde and then embed them in OCT (I always pat them dry before embedding). When I section them, I get...
12 February 2024 4,355 0 View
Can a black hole destroy matter and how does nitrogen get from the atmosphere to the biosphere?
25 January 2024 5,678 2 View
I am writing to request permission to utilize your tools in my Ph.D. thesis on the knowledge, attitude, and behavior of registered nurses toward pressure injury prevention. Your expertise in this...
20 January 2024 8,682 0 View
The cells are genetically engineered and grown on PDL plates for a potency assay. My BCA is highly variable. I am wondering if triton disrupts the PDL coating, which then interferes in the BCA...
11 January 2024 8,528 1 View