In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short... | Contact experts in PCR to get answers
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Questions related to PCR
SInce Notch is a cell signalling pathway, and yeast is unicellular, does notch exist in yeast?
02 October 2022 3,191 3 View
How to Assemble ITS 1 and ITS 2 Sequence for Isolate submission to ncbi ? I have already tried submitting by finding the similarity of my test fungal ITS Sequences with the ncbi blast, where...
19 September 2022 7,889 1 View
I want to measure antioxidant enzyme levels of SOD, CAT and GSH the problem is that the manuals from the manfacture are too difficult to understand
19 September 2022 9,605 9 View
I have experimented with alternative splicing in Exon 11 BRCA1, and I need to know how I can provide analysis and count my bp. We used two methods for gel, one regular and one automated by Qiagen...
12 September 2022 1,725 0 View
I need to do RNA extractions of a large number of samples from developing wheat grain, and plan to do them in the batches of 12. Is is sensible to keep the homogenised samples (with pestle and...
06 September 2022 7,855 1 View
Will really appreciate for any suggestions (references with synthetic procedure descriptions) on synthesis of fully substituted p-tertbutylcalix[4]arenes stabilized exceptionally in "cone"...
06 September 2022 7,463 0 View
I know that a T7 promoter can be added to the 5' end of the primers in PCR and RPA. I'm wondering if the same is also true for HDA or is there a way to get RNA products from a HDA reaction? (Or...
05 September 2022 1,088 0 View
I want to sequence various strains of Bacillus ssp with the PacBio Multiplex Sequencing Protocol. As you need 1 ug of DNA per strain, we use RotiPinkDNA (linear polyacrylamide with a dye) and...
26 August 2022 3,774 0 View
I have done a CA Techniques. I didnt have PHA so I was not added in culture setup. Therefore no metaphase was found my sample any possibility is available to get metaphase in sample pls let me know
10 August 2022 351 2 View
I ran the command gmx mdrun -v -deffnm npt_500 -rdd 1 in equilibration step. Then this error came called: Fatal error: There is no domain decomposition for 24 ranks that is compatible with...
05 August 2022 9,331 2 View
Hello, I have done a bit of research to see if there are any cells floating about in the colon (i.e. the lumen part) and cannot find anything that suggests there is, and would like to check if...
28 July 2022 2,992 1 View
I have been using DNASP to run analysis on my sequences, but with linkage disequilibrium the analysis does not run and I get the message in error(2).
22 July 2022 8,467 0 View
Hello! We would like to treat BMDM with the caspase-1 inhibitor Z-WEHD-FMK before bacterial infection. I only found two publications suggesting either 20 or 100 µM for primary macrophages. Of...
07 July 2022 3,910 1 View
As stated, I would like to know what is the difference between NCBI blast and EzBioCloud? I have sequences of multiple unknown bacteria and am trying to do the first identification on them...
20 June 2022 7,098 4 View
Hi all, I am struggling to transform my BY4742/1 cells using the Gietz protocol along with a linear PCR fragment. I understand that using a PCR gene disruption cassette is not as efficient as...
15 June 2022 7,202 3 View
Is it necessary to be in empty stomach of fasting( drinks) for at least 8-12 hours before lower abdomen USG? Or eating and drinking is allowed? why pressure in Urinary bladder (urination)is...
11 June 2022 8,155 10 View
I saw this structures on my primary culture of mesenchymal cells but idk what are those, does anybody knows what are those things?
29 May 2022 1,052 3 View
Hello, I received this SPR sensogram for small molecule binding affinity test on a protein. Would you please explain to me what are those dotted lined next to solid line? Thanks
20 May 2022 1,183 3 View
I want to expose my lung epithelial cells to different treatments in vitro and then detect pro- and anti-inflammatory cytokines/chemokines in the conditioned media - can I then do PCRs on the...
19 May 2022 2,637 4 View
I found an exon skipping event by RT-PCR I want to assess whether exon skipping changes the reading frame? And is this variant translatable? Is there any method or software to detect it?
19 May 2022 9,442 2 View
Recently, we have done the macrophage antigen lpresenting experiment by co-culture of macrophages pre-loaded with OVA and CD4+/CD8+ T cells isolated from OT-II/OT-I mouse spleen. Using CFSE to...
11 May 2022 1,711 6 View
Hello, I'm trying to clone my amplified insert in pGEM-T vector using T4 DNA ligase and not getting positive clones even after trying variations in vector to insert ratio. Amplification of insert...
22 April 2022 960 2 View
I picked a primer sequence ,designed by the author, for my PCR from a published paper. The issue is: UCSC Insilico Pcr is not browsing that sequence, although a detailed study is done on these...
18 April 2022 8,110 6 View
I have outsourced Sanger sequencing of my purified PCR products. Looking at the chromatogram, I know to trim the ends. However, the ends that need to be trimmed according to the quality scores are...
08 April 2022 7,551 3 View