In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short... | Contact experts in PCR to get answers
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My three journal publications and one conferrence abstract regarding subacute stroke recovery and neuromodulation are verified as someone's works before I registered on ResearchGate. I and the...
3 weeks ago 3,769 2 View
Global access to technologies to support safe and effective inguinal hernia surgery: prospective, international cohort study
3 weeks ago 4,307 1 View
we use F18 -PSMA 1007 for diagnosis of prostate cancer patients who will most benefit from Lu PSMA therapies there any difference noticed between it and Ga 68 PSMA 1007 as mentioned in the literature
last month 2,932 0 View
Thank you for all your kind helps! 1. Spiridonov, S.E., Ivanova E. S. 1998. Parasitic nematodes of tropical megascolecid earthworm Pheretima leucocirca from Ba Vi National Parc in Viet Nam....
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Does anyone know of any open access WSI database of thyroid cytopathology , specifically PTC, ? Urgently needed. Thanks
04 July 2025 3,919 0 View
In simple words, I am just asking that how Microbial Colonization contribute to the development of Burn wound infections or what role Microbial colonization play in the progression from burn wound...
30 June 2025 1,217 2 View
We are working on formulating a complex semi-defined medium to support the growth of a diverse, gut microbiome–like community. The original recipe we are referencing includes “digested serum” as...
26 June 2025 3,767 1 View
I’m using the Verso cDNA Synthesis Kit (Catalog number AB1453B) to synthesize cDNA for real-time qPCR. Could you please suggest an ideal RNA input amount for best performance? Thank you.
17 June 2025 1,878 1 View
I want to compare the expression of two genes (Gene A: 300 bp amplicon, Gene B: 200 bp amplicon) using SYBR Green qPCR. Is it valid to directly compare their expression levels given the difference...
16 June 2025 2,744 0 View
Hello All, I'm in the qPCR validation step of RNAseq gene expression and my qPCR calculated logFC is showing as upregulated for all my test genes, including predicted downregulated genes. This is...
12 June 2025 863 1 View
I am purifying and enzyme but, I am worried if I use some much energy affect the activity of the enzyme. How much energy from the sonicator, is safe to keep an purified enzyme with high activity,...
27 May 2025 5,273 3 View
Need an appropriate perineometer
27 May 2025 5,399 1 View
I have been having a hard time figuring this out. I would appreciate anyone who can help. Thank you so much!
24 May 2025 8,862 0 View
I’ve synthesized ZnS nanomaterials for phosphorescence studies and am now trying to measure their photoluminescence (PL) quantum yield using a spectrofluorometer. However, I’m struggling to...
15 May 2025 4,340 2 View
The bioinformatics and computational analysis predicated epitope now should i confirm the predicated epitope in wet lab or need to check the whole protein?
08 May 2025 9,999 0 View
Dear friends, Together with colleagues, we are conducting research on the morphological consequences of elytra loss in beetles (see our recent study in Systematic Entomology:...
17 April 2025 9,266 1 View
The logistic regression output for the JMP statistical package reports separate Chi square values and separate p-values for each independent variable in the form of Likelihood Ratio tests and as...
16 April 2025 1,149 4 View
Hi! I am trying to use DCs for infection assays, I start the process by rapid thawing frozen PBMCs (previously isolated using ficoll from human buffy coats), then using a kit for cd14+ monocyte...
13 April 2025 9,159 5 View
These are the articles required by our team for the systematic review: 1. Efficacy and safety of oral Fosfomycin Trometamol powder for the treatment of urinary tract infections caused by...
10 April 2025 2,790 0 View
Hard goat cheeses ripen with the participation of mites on the surface of the rind. Their number can serve as an indicator of safety. This requires a fairly simple and effective technique suitable...
05 April 2025 9,887 0 View
I tried using NanoAnalyze but i observed that it does not fit the data correctly. Please suggest me which software can i use for isothermal titration calorimetry data fitting as an alternative to...
31 March 2025 1,499 3 View
How do I publish a research paper without affilation? All related (59)
24 March 2025 1,361 1 View
Hi, I am collecting RP-HPLC data using Shimadzu Labsolutions Version 5.71 SP1. Every time I do the postrun PDA analysis, I have to manually remove unwanted peaks, e g. below retention time=4.5...
19 March 2025 3,767 7 View
as this was a part of my college assignment and i need to give submit it to them
13 March 2025 362 1 View