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Questions related to DMSO
Dear All Does anyone know which is the best media to freeze exosome isolated by ultracentrifugation? PBS only or PBS with DMSO many thanks for help
06 June 2016 1,168 3 View
Im trying to synthesize sols containing barium, which will then be converted into fiber.. problem is only barium nitrate dissolves in DMSO, but DMSO seems not to be very suitable for...
06 June 2016 4,419 1 View
We are having a debate in the lab. Protein lysates are wanted from these frozen cells to run a cell signaling luminex assay. I say if you are going to do this, you should plate and culture the...
06 June 2016 1,990 4 View
I want to prepare amyloid stock solution in DMSO. Article says sonication for 15 minutes at RT. they not mentioned intensity, I am confused, as this much long may be warm solution too much.
06 June 2016 6,874 3 View
Assuming the use of 90%FCS+10% DMSO as cryoprotectant and 1ml of final volume. Thank you in advance
05 May 2016 4,276 3 View
Two to five gallon batch processing is sufficient. A refrigerated condenser is preferred so that cooling water is not required.
05 May 2016 2,865 1 View
Recently we are facing this problem. We are using 20%complete medium + 10%DMSO or 90%FBS+ 10%DMSO as freezing mixture. None of these freezing mixtures are freezing even at -20. we are sure our...
05 May 2016 3,318 6 View
I am study a segment of a membrane protein, but it is difficult to dissolve in water. I was told to dissolve it in DMSO. It did work, but I worry if DMSO denatures protein?
05 May 2016 6,880 4 View
I have a problem because when I add doxycycline (in distilled water, DMSO, methanol or PBS according to different protocol) at the doses (100-300) µm/ml to culture media (DMEM), it precipitates...
05 May 2016 713 3 View
I need a procedure of immersion the gold slide in DMSO.
05 May 2016 7,443 1 View
Researchers in essential oil research
05 May 2016 7,081 2 View
i want to synthesize organic azide in this form N3-CH2-CN. I dissolved sodium azide in DMSO then added chloroacetonitrile. After addition i got yellow solution then did liquid-liquid extraction,...
04 April 2016 7,045 2 View
I am using DMSO as a vehicle for dissolving the drug for the the topical application on wounds. Dose anyone have observed the adverse effect of DMSO on wound healing or other side effects?. What...
04 April 2016 4,342 5 View
Whether Signal to noise ratio of 1H NMR spectra will reduce with increase in ionic strength. Like maleic acid in D2O will have less S/N than in DMSO. If so Please share is there any method...
04 April 2016 6,900 5 View
I am looking for some insight as to how to improve my yields from performing an MNC extraction using Ficoll for cord blood units that have been cryopreserved. The main issue is the cryoprotectant...
04 April 2016 4,728 2 View
I recently found a new DNA ladder assay Suman S, Pandey A, Chandna S. An improved non-enzymatic “DNA ladder assay” for more sensitive and early detection of apoptosis. Cytotechnology....
04 April 2016 925 0 View
I dissolved 100mg of biotin in 1000uL of DMSO. I take 10uL of this and biotinylate cells in 1000uL PBS. This means I have 1% DMSO. Would this cause cells to be lysed?
04 April 2016 5,872 1 View
I want to disolve drugs in DMSO and want to apply them topically on wounds, but some researchers have pointed me that it is cytotoxic even at lower concentration i.e. 10% or more. Can anyone...
04 April 2016 1,465 8 View
I have some problems to use camptothecin. The first time I prepared a dilution in DMSO and it worked. The second time I used the dilution stored at -20°, but it didn't work. So I prepared again a...
04 April 2016 9,417 2 View
Hi everybody, I'm trying to dissolve my EOs in an organic solvent, I choose DMSO 2% but my EO (Citrus peel EO) does not dissolve and they made an opaque solution(image attached). There was no...
04 April 2016 8,920 5 View
Hello, I want to ask a question regarding the inhibitory action of DMSO with AChe. If DMSO are showing inhibitory activity against AChE, and we supplied drug by using DMSO as a vehicle. Then how...
04 April 2016 3,311 9 View
In my assay, the substrate contributes a lot of DMSO, about 3.2% at a 100uM and contributes 0.8% making a total of 4% DMSO concentration in the assay. So we decided to make the DMSO concentration...
03 March 2016 2,824 6 View
I am trying to oxidize alcohol into an aldehyde. I have tried reaction with DMP. I could neither trace starting material (SM) nor product. So I gave though about DMSO oxidation. I tried...
03 March 2016 6,386 4 View
May I store it in aliquots at -20C°?
03 March 2016 3,887 4 View