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Questions related to DMSO
I am using DMSO treated SH-SY5Y cells for negative control but i get high expression of protein in it. I treated my cells with different toxic drugs to induce Histone modifications but. My...
17 October 2018 7,404 1 View
Hi all, I am trying to implement the perforated patch-clamp method to assess intrinsic excitability in mPFC slices from adult rats (PND 70-90). Maintaining enough positive pressure in my...
09 October 2018 999 3 View
I read Clinical Standards and Laboratory Institute standards where they permit use of ethanol/methanol/acetone for some antibiotics which can't be dissolve by water or DMSO. If some antibiotics...
03 October 2018 9,394 7 View
I am trying to formulate an ink that is composed of PMMA dissolved in anisole and some percentage of DMSO. My typical inks have been using 120kDa PMMA at a concentration of 40mg/mL in anisole with...
03 October 2018 9,892 2 View
I have not encountered this problem before but for a period of 2 weeks I'm getting different optical density values when I read them just after 10 seconds. I agree with the concept of color...
01 October 2018 5,460 7 View
A graduate student recently asked me this question and I stumbling to give a good answer. We freeze our purified proteins quickly (liquid nitrogen or dry ice/EtOH) to prevent ice crystals from...
12 September 2018 5,729 3 View
I'm trying to get a molecular weight for my polymer which is supposedly composed of 200 glycerol monomethacrylate monomers (GMA) with molecular weight ~32K by using MALDI-MS. I'm not sure what...
11 September 2018 4,506 3 View
I am not sure whether an hour of incubation will be enough to kill all the cells
04 September 2018 4,601 3 View
Hi, I ran an MTT assay with 7 doses of my drug ( dissolved in DMSO , final concentration in media ,0.05%) , and read the absorbance at 490nm according to the reagent manufacturers instructions....
03 September 2018 5,176 3 View
Hi, I culture AGS cells human gastric cancer cells. I use materials 'flavonoid' for inhibiting cancer cells. I use DMSO for solvent, 0.5% of total volume in RPMI. Anyway, i treated with various...
30 August 2018 444 8 View
1.What is the maximum number of daily IP injections and 2. The maximum volume (per day & cumulated) tolerated in mice with a saline media (hydrophilic compound) and with DMSO (non-hydrophilic) ?
23 August 2018 3,957 1 View
I want to calculate the solvatation effect of DMSO on the UV-vis spectra of some molecules with PCM Model using TD-DFT with the 6-311++G (d, p) basis set. Regards Nadjib CHAFAI
10 August 2018 4,058 2 View
I have been searching for a different way of determining the chlorophyll content in dragon plant (pitaya) cuttings without the use of Dimethyl Sulphoxide (DMSO) or any other chemical.
07 August 2018 5,274 3 View
In the process of making a 5 mM stock solution of rosiglitazone dissolved in DMSO, I used a non-DMSO resistant filter. The DMSO essentially disintegrated the filter, and the debris contaminated...
01 August 2018 4,681 1 View
Hello everyone I have dissolved organic compound in 100% DMSO in high concentration to check its biological activity on cancer cells after diluting it in media. Once it was added to media for...
28 July 2018 8,259 5 View
I have dissolved lovastatin into DMSO for its loading into hydrogels. How can we remove DMSO from finished product?
24 July 2018 5,596 3 View
Hi, I am trying to dissolve pure reagent grade Quercetin into cell culture medium (DMEM+sodium bicarbonate+antibiotics) but it clearly does not form a solution. I've found that DMSO might serve...
23 July 2018 4,113 3 View
I am trying to measure the fluorescence spectra of pyrene molecules at excitation wavelength of 310 nm with emission scanning wavelength 350-450nm. But, getting broad spectrum instead of two or...
16 July 2018 6,435 8 View
Has anyone used rupintrivir (PZ0315) from Sigma? the rupintrivir powder was recommended to dissolve in DMSO and stored at -20 degree. I am wondering if anyone knows how long the aliquot of...
12 July 2018 9,495 2 View
After their culture, the cyanobacteria reacted with Bennett Media to produce their compounds into the supernatant preserved after centrifuge. They form a homogeneous mixture for more than one...
09 July 2018 7,554 2 View
I am planning on making working solutions of 17-beta estradiol in DMSO. The drug has been exposed outside of the culture hood. My studies will last up to 7 days, so I am wondering if I should...
09 July 2018 8,022 4 View
I performed MTT assay for assessing the cytotoxicity of curcumin (0-50uM) on LPS-stimulated THP-1 macrophages, and used DMSO as control vehicle. While calculating the cell viability% for DMSO, I...
27 June 2018 8,461 6 View
I need to use both Hydroxytyrosol and Oleuropein powders (from Sigma Aldrich) to underly in vitro studies in cancer cells. Data sheet doesn’t provide further information about storage once the...
25 June 2018 8,038 4 View
Dear all, I would like to ask, if DMSO can be a positive control in this particular experience. L929 murine cells stimulated by murine-TNF-Alpha by 8 hours mainly leads to cell death by...
22 June 2018 2,552 2 View