Are there any Cryopreservants/Cryoprotectants with no DMSO?

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Farjam Goudarzi

Glycerol is also used as a preservative. Highly viable cells are achieved.

Nafise Amiri

Yes glycerol is a good choice. Check the links below, they may help :

Article Cryopreservation of Endothelial Cells in Various Cryoprotect...

http://tools.thermofisher.com/content/sfs/brochures/D19575.pdf

Jean Carlos Bettoni

Yes, PVS3 vitrification solution; PVS3 contains 50% glycerol (w/v) and 50% sucrose (w/v) in basal medium.

In these 2 paper attached there is information about PVS3: NISHIZAWA, S., SAKAI, A., AMANO, Y. AND MATSUZAWA, T. Cryopreservation of asparagus (Asparagus officinalis L.) embryogenic suspension cells and subsequent plant regeneration by vitrification. Plant Science, Limerick, v.91, p.67- 73, 1993.

SAKAI, A.; ENGELMANN, F. Vitrification, encapsulation-vitrification and droplet-vitrification: a review. Cryo-Letters, Kew, v.28, p.151-172, 2007.

With Best Regards,

Jean