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Questions related to DMSO
Please share any literature regarding this study if it has been published.
28 March 2018 8,645 2 View
I am looking to ship ~600 tissue samples in the form of mammal ear clips from the UK to the US. These samples are currently in a high % ethanol solution, which is considered hazardous and thus...
27 March 2018 8,896 2 View
I am using an aTC inducible TetR expression system, and so wish to use aTC for protein induction in E.coli HM0079. I know that when dissolved in water, aTC stock is only stable for around 24...
26 March 2018 4,800 4 View
Hello everyone! I have tried to dissolve MGITC (Malachite green isothiocyanate) in DMSO but, it was not stable in the solution. I couldn’t make the stock solution. Could Someone tell me what...
22 March 2018 2,735 3 View
recently I did experiment in culture cell treated with Curcumin. I made a stock of Curcumin with concentration 10 mM from 36 mg of Curcumin powder in 10 ml of DMSO. i treated my cell with...
06 March 2018 3,304 3 View
Hi guys, I learned that dmso peak in 1H NMR caused by proton exchange. So the peak at 2.5 ppm indicates dmso-d5 not d6 Also the dmso which have more exchanged proton like d4 or others can be shown...
05 March 2018 9,502 3 View
Dear all, Does anyone know or have an experience in freezing (cryopreservation) of the primary Human Hepatic Stellate Cells? How much DMSO is recommended to use? I read that cryopreservation of...
04 March 2018 9,991 2 View
what is the acceptable limit of DMSO percentage in MCF-7 and MDA-MB-231 for dissolving compounds? i am using 0.2 % DMSO for Annexin V/ PI assay, TUNEL assay and immunocytochemistry and getting a...
02 March 2018 9,751 9 View
I am trying to induce toxicity in hepg2 cells by ccl4.According to paper"HepG2 cells were incubated in medium or treated with the toxic agent (20 mM, 30 mM, or 40 mM CCl4 in 0.05% DMSO".When i am...
24 February 2018 7,603 1 View
I have been trying to freeze-dry a solution of Diallyl trisulfide (DATS) to remove the DMSO in order to analyse DATS via HNMR. However, I am getting very little DATS. I am thinking that DATS could...
20 February 2018 3,214 2 View
I am using A549 cell line and i had made stocks in Nov 2017 and stored in -80. When I revived them back they are growing at a very slow pace. As doubling time in 22 hrs approx.But in this case I...
19 February 2018 3,166 2 View
I've recently begun working with RA in my media for differentiation of primary airway epithelial cells. I need to supplement RA into the media when I change it ~every other day. I currently have...
06 February 2018 9,148 1 View
I need to conjugate PLGA with carboxylic end and peptide (1061 Da). The peptide has two unprotected NH2-groups. When using EDC and NHS (PLGA:EDC:NHS = 1:5:5, molar ratio) the percentage of...
06 February 2018 2,664 3 View
I had prepared lanthanide metal complexes from schiff base of carboxylic acid and they are not soluble in common organic solvent. they are partially soluble in DMF and DMSO. Please suggest the...
20 January 2018 7,422 3 View
I'm doing OGD experiment for mature oligodendrocytes cell lines and it's not showing an expected amount of cell death and apoptosis (damage was not significant compared to the control). I’ve...
28 December 2017 4,746 3 View
Hi, The storage temperature for Latrunculin A (waxy solid) is -20 deg. C whereas storage temperature for DMSO is 2-8 deg.C. After I dissolve the latrunculin A in DMSO what temperature should I...
01 December 2017 10,002 1 View
I want to prepare an aqueous solution of Reserpine to test it's MIC. I've tried to use 8% DMSO as the solvent (which will give me final conc. of 2% DMSO during the assay) but Reserpine...
01 December 2017 6,773 2 View
Sorry I am not a chemist, but need a simple (references or explanation) about what is the advantage(and disadvantage) of choosing DMSO or DMF when fabricating a perovskite solar cell. It is known...
27 November 2017 6,687 13 View
Hello, I was told that I had to refrigerate my DMSO for storage purposes, I plan to use mine for transdermal application of a plant based molecule. I took it out of fridge today after noticing it...
22 September 2017 6,818 1 View
I am working on an e-beam process that defines metal gates with extremely high sensitivity to the repeatability/resolution of the defined geometry. I am looking to improve our recipe since we...
23 August 2017 1,160 4 View
We use a 1mg/ml stock solution of JC-1 in DMSO. We add this to the medium to achieve a final concentration of 5ug/ml. However, we notice a lot of precipitates of JC-1 in the well, even after 3min...
20 January 2017 4,181 4 View
I generated a line of HeLa cells which stably express an EGFP plasmid. After going through the process of selecting and growing a colony, the cells were passaged 10 times to ensure their...
17 October 2016 575 2 View
The injection volume of the sample is just 2uL. The sample does not dissolve well in water/100% methanol/or mixture of water-methanol. The sample dissolves well in DMSO.
07 July 2016 4,145 2 View
I need to dissolve vitamin d in least possible volume of dmso not to kill the cells. But I need total volume of 1200 up to reach molar conc of vitamin d. Of this 1200 ul, what percent of dmso can...
06 June 2016 4,632 1 View