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Questions related to DMSO
I am working on PAN/DMSO needleless electrospinning with 10wt% concentration and I am facing problem that fibers made are not depositing on the aluminum foil and they are hanging like web in the...
31 October 2019 3,170 5 View
Dear all, I have been trying to use a pgr106 vector for cloning work. However I am facing many issues when I am trying to digest it in the MCS region. The sites I have selected for cloning is cla1...
30 October 2019 8,598 4 View
I'm currently isolating a number of Alternaria solani and A. alternata strains from leaf samples and am considering my options for long-term storage of both mycelium and spores. I've read...
30 October 2019 6,118 3 View
The NMR of a series of novel compounds synthesized using identical procedure on different time intervals -all- showed a strong single peak at around 8.46 in proton NMR using DMSO-d6 or MeOD, the...
23 October 2019 6,563 2 View
Could you kindly inform what are the best concentrations of 2',7'-dichlorofluorescein diacetate stock (in DMSO) and working solutions (in PBS) for detecting ROS generation in leukocytes by flow...
22 October 2019 5,330 3 View
Dear all Molecular Biologist, I am trying to prepare competent cell for transformation. but i am confused to use glycerol or DMSO as a cryopretectant. there is any...
20 October 2019 3,006 5 View
I tried to thaw my HaCaT cells several times but 99% of them died. They were frozen with 10% DMSO + 10% FBS + 80% DMEM. I also tried 10% DMSO + 90% FBS then they died less but still only 10-20%...
19 October 2019 1,985 2 View
It is reported that PES/DMSO membranes are too brittle. Is there any method or additive to make them more flexible? Regards
19 October 2019 1,873 6 View
Hello Just wondering when I stimulate the HL60 cells with DMSO and use them around D7, do you advise to change the medium just like how we normally pass down the cells? Or should I keep them in...
08 October 2019 7,076 2 View
I received SH-SY5Y WT cells and started growing them in low glucose DMEM with FBS and S/P. After reading article about changed cell morphology and detachment of cells from growth surface due to...
07 October 2019 1,756 2 View
Hi, I have some problem with the viability of my primary epithelial cells after thawing. I have used two protocols (centrifuging or only diluting 10 times more and changing the media the day...
04 October 2019 8,248 6 View
When validating a new Planer Integra 750 plus we observed very slow engraftment for the first 5 patients compared to other prior patients receiving stem cell grafts frozen with our old Planer as...
02 October 2019 4,035 1 View
during lift off process for introducing metal contacts to my graphene device, I used DMSO instead of acetone but after the process my graphene was completely destroyed. Does anyone have an idea why?
26 September 2019 2,066 2 View
My lysis Buffer contains 5M Nacl, 0.5M EDTA, 1M tris ph 10, 10 MNaOH, Then 1% triton and 10% DMSO is added on the day of the experiment. Electrophoresis Buffer contains 0.5M EDTA, 10M NaOH and 1%DMSO
25 September 2019 7,234 1 View
What is the best condition and solvent to attach acyl halide ( methacryloyl chloride) derivative of carbamide(allantoin)? So far I tried with the following solvents DMSO, MeOH, DMF, DCM. But no...
20 September 2019 7,954 4 View
Hi everyone, I just want to know that it is any problems if i use GC enhancer or DMSO with my qPCR? Does anyones give me some advices? Thanks a lot.
18 September 2019 1,779 1 View
I want to remove DMSO from a solution, without heating above 70C, DMSO we used to make solution with PANI.
16 September 2019 5,035 5 View
I wish to conduct an in vitro assay of rosmarinic acid and caffeic acid against fungal pathogens using Czapek-Dox liquid media. In this case, between ethanol and DMSO which solvent will be better...
06 September 2019 1,096 10 View
I am currently working on a project where an animal experiment has been conducted. This was a stress experiment where the "repeated social defeat"- paradigm was used. I want to do some analyses...
27 August 2019 1,942 2 View
the nanoparticles were purchased from sigma and they have a size range (35-50 nm). i tried to dissolve it in DMSO ( 5% of the nanoparticle solution) and i also tried to sonicate it for 30...
25 August 2019 9,912 10 View
I extracted bioactive compounds from garlic by using n-hexane and evaporated it afterwards. However, the concentrated extract is not fully dissolved as I can see precipatates floating in...
23 August 2019 2,123 12 View
I am attempting to amplify a 15.4kb sequence from genomic DNA. The DNA template is high quality (fragment size >80kb) and I'm using the Generuler HR ladder. Gel is 0.4% agarose. I'm using the...
05 August 2019 8,944 2 View
We have frozen (10% DMSO, 90% FBS) spleen cells which we want to thaw and enrich for CD8+ cells. Can anyone recommend the best way to thaw theses cells - would you use dnase treatment, and what...
27 July 2019 5,586 2 View
I am doing my thesis on Perovskite solar cells. I am trying to prepare triple cation cells, but the color of the cells after deposition is milky, which I think would be due to wrong solution...
24 July 2019 9,311 2 View