How can i prepare an optimum HUVEC freezing media?

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Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

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Federico Galvagni

We use 90% ordinary FBS + 10% DMSO with good results.

Cássio Prinholato da Silva

The same of Federico!

Or you can use glycerol instead DMSO, but DMSO is better.

د.باقر فليح عبد الحسن

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Hans Rajh

we are using regular culture medium with 10% DMSO, it is giving good retrieval rate