How can i prepare an optimum HUVEC freezing media?

More Mehmet fatih dağlı's questions See All

SAP2000 Hinge PMM Surface Not Convex ?

I have modeled bridge pier. I have assigned plastic hinges to circular piles and oblong column. I am getting error only in oblong column hinge as PMM surface is not convex. I am not taking any...

14 May 2024 8,820 0 View

What do you think about using AMBER99SB charge when docking with peptides using Autodock Vina?

I have 1215 peptides between 4 and 13 mer in length. I want to dock them with peptides using Autodock Vina, but I used AMBER99SB instead of Kollman charge to give better and reliable results. I...

11 May 2024 8,014 2 View

How can I improve the efficiency of single gRNA to hybridize with Cas9 to get the RNP complex?

We use synthetic gRNA from IDT but we think have some problems for sgRNA hybridization with Cas9. any suggestion is welcomed!

25 March 2024 1,536 3 View

I am looking for a teammate for an article I will write about gastronomy tourism?

I'm thinking of starting an article about gastronomy tourism soon. For this reason, I need a teammate who can offer different perspectives and produce quality publications and is suitable for...

04 December 2023 8,403 6 View

The Strength of Granules and Agglomerates Author: H. Rumpf Publication Year: 1962 is this resource unavaliable?

I've searched various academic databases and online platforms but have come up short of accessing the full content. If anyone has a copy or knows of a way to access this paper, I would be...

22 October 2023 7,514 0 View

How Lignin, Cellulose, and Hemicellulose is related to biomass combustion fuel properties?

Hello, researchers of biomass. I believe many of you possess invaluable insights on this topic. Beyond basic introductory content, I am keenly interested in sourcing comprehensive resources that...

22 October 2023 4,889 1 View

If a journal is in different quarterlies in two different fields of the WoS classification, which one should we consider?

For example, a journal to which I am considering submitting a manuscript is included in different quartiles (Q2 and Q3) of WoS in both environmental sciences and sustainable science and...

09 October 2023 7,927 4 View

How can i interpret this QSAR graph (pEC50, training set and test test) ?

Hello everyone, I use ChemMaster for QSAR modelling. Firstly, i calculated pEC50 and finded Molecular Weight, rotatable bonds, LogP. Then, with Kennard- Stone Algorithm i divided the datasets...

27 September 2023 5,862 1 View

Two digestions of the same sample give out different results in ICP. Possible reasons?

Dear colleagues, I'm currently working with ICP-OES, and we've been doing twin digestions of the same sample for crosschecking. From time to time, I get significant differences between results...

09 July 2023 5,399 6 View

I am seeking of NZ2 strain ORFV virus. Is there the virus in your labs collection?

Dear Researchers, I need 2 ml of NZ2 strain ORFV virus for my student Ph thesis. Could you send us, buyer pay. My address: Prof.Dr. Mehmet KALE Department of Virology Faculty of Veterinary...

29 May 2023 7,105 6 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

Cell proliferation assays suggestions?

What cell proliferation assay do you suggest? cyQUANT from thermo Fischer has good reviews, a lot of good articles for the kit from Abcam. and Promega kits look easy and straight forward.

21 July 2024 1,554 2 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

19 July 2024 8,522 0 View

Kraft lignin and MMA(Methyl meth acrylate) free radical polymerization for synthesis of thermoplastics?

I am conducting free radical polymerization using Kraft lignin and MMA, with DMSO serving as the solvent and there is also an initiator. Following the reaction, I obtain a solution, but I am...

15 July 2024 9,827 1 View

Regarding solubility of Dorsomorphin in DMSO ?

I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...

11 July 2024 4,832 0 View

To what degree does the immunohistochemical (IHC) evaluation of Ki-67 hold diagnostic value beyond neoplastic pathologies?

In the context of doctoral research, I utilized immunohistochemical (IHC) analysis to quantify the expression levels of the Ki-67 proliferation marker. While the application of this test and...

25 June 2024 7,825 2 View

Does anyone have suggestions for this esterification?

I have a very polar alcohol (soluble in MeOH, H2O, partially in heated DMSO). And a long chain fatty acid that I would like to couple. I can't find a common solvent for them. I can form the...

24 June 2024 9,319 0 View

Medium replacement interfering with cell culture blot results?

We utilize an immortal endothelial cell line and various agonists to observe their effects on Src phosphorylation. However, our protocols seem to induce high basal levels of Src phosphorylation....

17 June 2024 1,649 0 View

Problem with nucleofection RNP delivery to murine primary T cells?

I have been using the Lonza nucleofector for RNP delivery to OT-I T cells without any issues for the past six months. However, recently, my nucleofected OT-I T cells have not been proliferating in...

13 June 2024 2,279 0 View

Federico Galvagni

We use 90% ordinary FBS + 10% DMSO with good results.

Cássio Prinholato da Silva

The same of Federico!

Or you can use glycerol instead DMSO, but DMSO is better.

د.باقر فليح عبد الحسن

آسف السؤال ليس من اختصاصي

Hans Rajh

we are using regular culture medium with 10% DMSO, it is giving good retrieval rate