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Questions related to DMSO
Dasatinib is an inhibitor of tyrosine kinases. I would like to test the effect of Dasatinib in vivo on my T cells. However, Dasatinib has a very low solubility (0.5mg/ml) with PBS, which is...
22 July 2019 3,151 3 View
I am trying to load an isoflavonoid which is hydrophobic in nature onto graphene oxide by simply mixing a 1:1 aqueous dispersion of graphene oxide with the isoflavonoid mixed with DMSO (Compound...
09 July 2019 8,476 3 View
Dear Researchers, I have little problem, i want to know only this kind of problem with me, or also other researcher face same issue I am preparing CsPbI2Br perovskite with precursor CsI+PbI2+PbBr2...
06 July 2019 5,760 7 View
I do MTT assay for cell viability. I used MTT solution 1mg/ml, 2h incubating and then treated DMSO to formazan. I read 595nm. In march, O.D was fine ( repeated 6 times ). So i perform...
05 July 2019 7,034 4 View
Hi, i would like to know will the humidity (40-60%) of the room be detrimental to the MAI powder when weighting on the analytical balance? is the moisture sensitivity reduced when MAI is quickly...
26 June 2019 5,767 4 View
During synthesis and final collection of compounds, how to remove high boiling solvents like DMSO, DMF from final compounds.
24 June 2019 941 3 View
Hello everyone, I recently thawed THP-1 cells, which were frozen at passage 12 in 2016. I got the cells back to culture in RPMI + 10% FCS + 1% HEPES + 1% PenStrep. The cells seemed to recover...
20 June 2019 7,898 6 View
Here is the structral formula of the polymer Amine poly(ethylene glycol)-block-poly(lactide-co-glycolide)
12 June 2019 6,863 8 View
I am currently doing MTT assay on MDA-MB 231s and I treat the cells with 0.2% of DMSO (vehicle control). the problem is that the DMSO seems to increase cell viability (above 100% as compared to...
09 June 2019 5,933 2 View
How to characterize the stability of DMF ( N,N-Dimethylformamide ) or DMSO ( Dimethyl sulfoxide ) solution of Lead Iodide? Can you give me some advice or references? THANKS
07 June 2019 6,147 1 View
I am extracting RNA from 10%DMSO+FBS, cryopreserved PBMCs. I am performing RNA seq. Although I am getting decent RNA yield, it has come to my attention that the DMSO may affect the RNA quality....
04 June 2019 10,033 3 View
I am trying to evaluate the toxicity of ZnO-NP in aquatic organisms, but we have not improved its dispersion by using DMSO. Acidification (p/e: pH less than 7 or 8) is not suitable. How can I...
01 June 2019 9,054 2 View
I am trying to sort out this primer dimer (in NTC) issue for a very long time. After many of my previous trials in which NTC and my sample bands looked same, only in this trial the NTC and my...
30 May 2019 6,981 2 View
I've mistakenly put the cell that i wanted to freeze in the Room temperature overnight with 10%DMSO & 90%FBS. I wonder if the cell can still be alive?
28 May 2019 5,296 1 View
I am trying to do experiment with mixing PEDOT:PSS and silver nanowire(AgNW). However, when I search the product of AgNW, it is produced with solvent such as ethanol, water, and IPA. And I found...
23 May 2019 7,990 1 View
Actually i want to know how to make freezing medium with 10%DMSO& 90% FBS?What should be the condition to keep it and how long time is the shelf-life?Much tnx for your suggestions.
21 May 2019 7,433 3 View
Hi, we're working on a pilot research and our aim is to develope parkinson dis. with both intranuclear and intraperitoneal injection methods. In i.p. model The problem is rats can not tolerate the...
18 May 2019 9,838 3 View
I prepare cells for storage according to ATCC guidelines, usually 10% DMSO and 20% FBS. Cells are in cryostorage vials intended for use in liquid nitrogen. Cells are Hek293T, HepG2, HeLa and...
15 May 2019 7,927 3 View
The image shows 3 technical replicates overlayed of 8uM of the his-tagged protein eIF4E in LCB buffer (20mM Hepes pH 7.5, 0.2mM EDTA, 100mM KCl). The other image shows 3 technical replicates of...
11 May 2019 7,653 3 View
Hello, I typically use 3:2 DMSO:Water for cyrogenic wet sectioning, but I have a material I need to section that dissolves in DMSO. What, other, ideally non--polar, liquids are good for wet...
10 May 2019 1,258 1 View
I have some patients cryopreserved lymphocytes samples which I want to analyze with RT-PCR later. It has been stored in the liquid nitrogen tank more than 2 years. I also want to use them for...
17 April 2019 492 4 View
I have been trying to make solution of Guanine ( C5H5N5O : 2-Amino-1,7-dihydro-6H-purin-6-one). Following are the solvents I tried for Ethanol, Ether, chloroform, DMSO (dimethyl sulfoxide),...
12 April 2019 9,341 3 View
Can Amyloid-beta peptides be stored in DMSO and froze in -20C? If so, for how long?
10 April 2019 4,351 7 View
I want to make 5% DMSO solution for my substrate such as Fatty acids and naphthalene to dissolve.
05 April 2019 2,466 6 View