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Questions related to DMSO
After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .
01 March 2021 8,267 3 View
I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...
20 February 2021 7,955 2 View
I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...
19 February 2021 5,021 1 View
I resuspended my BCTC as described on the bottle at 10mg/mL in DMSO. However, I can see the formation of crystals which I can only assume are DMSO and that the drug is precipitating out of...
15 February 2021 6,109 1 View
When I do the test of plant against the free radicals of H2O2 the control had a value about 0.9126 The critical point is when I prepared the extract with DMSO the absorbance appear more than 2...
10 February 2021 6,843 2 View
Hello World, I have been working with LS174t. Complete Growth Media- 10% FBS/EMEM. ATCC says to freeze with 5% DMSO/CGM. when I defrost my vials, they are not attaching to flask. vials put into...
02 February 2021 9,021 2 View
Am functionalizing the metal nanoparticle with folic acid. in the process I have taken folic acid in DMSO and added DCC and NHS to activate the carboxylic acid group of folic acid in the standard...
02 February 2021 10,286 3 View
I have been trying to make an even PEDOT: PSS (PH1000) film with the doctor blade technique (tape casting) with varying results. I have been using a silicon substrate that's treated with piranha...
02 February 2021 9,006 5 View
I have a question about your letter, Fluorescent phosphoinositide 3-kinase inhibitors suitable for monitoring of intracellular distribution. Can you tell me how we can dissolve theophylline for...
27 January 2021 9,417 1 View
hello. i manually measured the area in each image. the output i got was area/mean/min/max then i put all the data into excel, and also added the following columns: group names/date/hour pic was...
20 January 2021 4,229 3 View
I wanted to get 0.2 mg/ml Ferrostatin-1 and 0.1% DMSO respectively. 10mg Ferrostatin-1 was dissolved in 50 µl DMSO first（in fact it was undissolved）, and diluted with 50ml 0.9% NaCl. Then the...
18 January 2021 2,162 4 View
Hello I want to know if I make a FITC-DMSO solution, how long time I can use it? Can I use the solution after a week? thanks for your help
09 January 2021 1,809 3 View
Besides, DMSO, EDTA, and K2Cr2O7 to scavenge the OH radicals, holes, and electrons respectively what are the other chemicals being used for scavenging? Please share the files with complete...
01 December 2020 5,228 1 View
I need to dissolve a few fatty acids of C10 to C20 such as myristic, stearic and arachidic acid for a PRO-DNA binding reaction to carry out an EMSA. I tried with DMSO, where I dissolved the acids...
30 November 2020 7,742 3 View
Hello! I am planning to get an alkoxide which should be potentially soluble in CH2Cl2. Do you think it is okay to use CD2Cl2 to get an NMR spectrum of the alkoxide? I am wondering if RO- anion...
26 November 2020 2,137 1 View
I am facing a problem regarding smearing in PCR amplification since two months. But, before six months the results was there using the same primers at 67.2-degree celsius annealing temperature, 5%...
24 November 2020 4,715 5 View
I actually want to give 2.5mg of AM281 in DMSO per kg of mice ? AM281 comes in the packing of 10mg by TOCRIS. https://www.tocris.com/products/am-281_1115 What concentration of AM281 should I make...
18 November 2020 3,810 2 View
Hello all. I am trying to isolate buffy coat from whole blood (sodium citrate), and planning on cyropreserving it for later analysis (~3-5 years). As to what analysis would include, we are leaving...
18 November 2020 2,197 3 View
I used DMSO to solve SB according to its data sheet.But it does not dissolve and settles as a yellow powder. And even despite heating at 40-56 C, it did not dissolve.Thanks if anyone has any...
18 November 2020 8,530 3 View
Hi, i'm trying to differentiate SH-SY5Y cell-line in neuron using retinoic Acid 10 uM. I prepared a 100 mM stock solution in DMSO (this is a right value to the optimal concentration range for...
08 November 2020 6,553 4 View
I'm currently working on developing a bioink with HMW HA-TA (hyaluronic acid/ tyramine) and Eosin Y alone. Final concentration of Polymer solution is 2.0 (w/v)% (in PBS) and Eosin Y (in DMSO)...
30 October 2020 9,133 2 View
Hello dear scientists: When I treated the Pc12 cell line with H2O2, after 2h the cells seemed to be dead. I didn't observe crystals, so I didn't add DMSO and left it with mtt in the...
21 October 2020 1,390 3 View
I added 2 mL of 0.1 M EDTA aqueous solution pH 6, to a solution of 3 mL pure DMSO and instantaneously I observed salt precipitation in solution. Is this happening because the pH of the EDTA...
18 October 2020 6,778 1 View
I have been running ELISPOTS with not much success recently due to high levels of background. After testing everything from the DMSO used in peptide stimulation to the FBS in the culturing medium,...
16 October 2020 1,201 3 View