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Questions related to DMSO
Dear researchers, We are trying to figure out a problem with our cells (caco2 and MDCK) and are hoping you can help us. The permeability of our control compound Nadolol is 10 times higher than...
19 June 2018 8,982 2 View
Cells are healthy when used routinely but once freeze downs are made (FBS + 10%DMSO) and stored in -80 C their revival is quite difficult and majority cells are dead. I am using RPMI-1640 media...
16 June 2018 6,156 3 View
I kept PbCl2 on the stirrer with DMF and DMSO separately, but I can't get a solution with them, just get the white crystals floating around that get deposited in some seconds. Any way I can...
16 June 2018 5,003 4 View
I found that DMSO that is used for MTT assay is freezed just for once by a person mistake? Does it effect DMSO (For MTT) efficiency?
13 June 2018 7,657 5 View
Hi all, We've been synthesizing some short (16 aa) peptides lately and they cleave beautifully from the resin, yielding a beautiful bulky white cloud in a 50:50 solution of hexanes/diethyl...
04 June 2018 820 2 View
Hello, has anyone experiences with the storage of PAHs dissolved in DMSO? How long can I store them and under what storage conditions (e.g. at 4, - 20 or - 80°C)?
23 May 2018 10,214 3 View
Should i use potassium iodide or iodine crystals or both of them?
10 May 2018 7,172 3 View
Hi, we are working in a very resource limited setting where the lab resources on site are very basic. We are doing serial blood sampling for drug concentrations, which includes overnight samples....
09 May 2018 1,015 11 View
MTT dye is reduced by oxygen reductase of the nucleus of living cells. Then Why does we add DMSO ?
09 May 2018 9,707 3 View
Will i get same morphology? In a journal they performed Solvothermal process to obtain CuO nanostructure. In this process DMSO is used as solvent of CuNO3. PVP and Thiourea is mixed. Solvothermal...
04 May 2018 9,697 2 View
I can´t manage to dissolve a solid Nitrogen-containing heterocycle-Polyoxometalate hybrid material to perform a liquid 31P nmr. I´ve tried: Solvent relative polarity CHCl3...
02 May 2018 3,846 4 View
I accidentally bought too much retinoic acid and concerned about how to storage it. I found out that I have to dissolve it in DMSO or Ethanol and keep it at -80C in aliquots. Would it be okay to...
21 April 2018 9,323 1 View
So I got it wrong and I added too little DMSO when I prepared my cell freezing media (100 µL DMSO to 4.9 mL of DMEM, instead of 500 µL DMSO to 4.5 mL DMEM). They are right now in a -80 ºC freezer...
21 April 2018 4,801 3 View
I used benzene as a solvent to extract the plant powder? I used water, 100% DMSO, 5 and 10% Tween 80, 5 and 10% Tween 20, ethanol, methanol. But the extract does not dissolved. other than these...
19 April 2018 2,150 3 View
Hello everyone. I started to work in a lab, where the focuses are molecular ecology and evolutive genomic. One of the projects is the genomic characterization of different samples of Pyura...
17 April 2018 2,961 2 View
I need to crystallize my metal complexes of cobalt, nickel, copper and zinc. What would be the best solvent to use? I read in the literature review saying it's either DMF or DMSO. Tried the DMF...
16 April 2018 5,798 4 View
DMSO is used as solvent for HNMR. Reaction of thymol and 4,5-dichlorophthalonitrile was held in DMF, after reaction completed it is precipitated in cold water. TLC and IR spectrum of product were...
10 April 2018 6,391 11 View
i followed two different protocols but my resistant starch sample does not dissolve easily in 90%DMSO and forms swollen granules due to which doesn't bind with iodine and shows lesser binding as...
07 April 2018 3,643 3 View
Hi everyone, I have been testing a Microfab print head on glass slides with PEDOT:PSS/DMSO solution mixtures and I have been noticing excessive spreading of the droplets upon deposition. Any...
05 April 2018 7,825 1 View
i have two different solid plant extract ethanol and water to check antiepileptic activity which is the best saline to dissolve the solid plant extract to i.p in mouse. thanks & regards...
04 April 2018 717 3 View
My compound is highly polar and doesnot dissolve most of the solvent, only DMSO is dissolve how to remove this DMSO any work up procedure??
04 April 2018 4,772 3 View
I am using luminol for ROS determination in Neutrophils. first of all i have problem in dissolution of Luminol. I made a stock of 3M in DMSO. Briefly I use 100 microleter of neutrophils from 5...
02 April 2018 5,922 2 View
hello , I ask for papers wich use acetone to dissolve plant extracts instead of DMSO thank you
01 April 2018 1,111 9 View
Can anyone explain why there is coloration of my PVDF films prepared by solution casting method using solvents DMAC & DMSO? I think it may be some sort of degradation of the polymer, I noticed...
30 March 2018 7,605 3 View