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Questions related to DMSO
Hi According to my research, I have to dissolve glibenclamide in DMSO to obtain a 10 mM stock solution. (I am adding it to a 10ml buffer solution in organ bath chambers for rat aorta rings)....
02 March 2020 7,142 2 View
Actually ,i couldnot evaporate DMSO due to high boiling point. So how can i get back my metal complex
02 March 2020 3,479 7 View
gas bubble generated when DMSO mixed with water in 50:50 concentration? having methane or n-butane like smell
18 February 2020 2,827 1 View
I'm facing a problem in dissolving two synthetic peptides (21 amino acids long). The peptides have iso-electric points at pH 4.01 and good predicted solubility in water. However, the synthesized...
17 February 2020 6,759 3 View
I used to test antiproliferative activities of chemical compounds dissolved in DMSO. I have been surprised when testing one of this compounds in HBL-100 cell line bacteria contamination appears. I...
14 February 2020 4,826 2 View
I'm making a boron-containing by-product which in only soluble in DMSO. I've been trying to make a crystal of that. I'm kind of sure is meta boric acid and I have a peak at around 20 ppm in B NMR.
06 February 2020 2,435 2 View
I noticed that these compunds are not soluble in DMSO, and DMSO damages hepatocarcinoma cells a lot. Thank you!
05 February 2020 6,795 1 View
The papers that I read on adipocytes mainly discuss to culture in DMEM+FBS+Penstrep and transferred into MDI Differention media for treatment. I would like to check the viability of the cells...
28 January 2020 4,442 1 View
Looking to design a protocol for freezing rat microglia, but have found little material in the literature. So far I have only found some papers recommending freezing in DMSO or glycerol for...
21 January 2020 3,226 1 View
We use a 0.5 mg/kg meloxicam for treating post surgery pain but this drug has been back ordered for months now. We have secured Meloxicam in powder form from Sigma (Catalog ID - PHR 1799)....
20 January 2020 7,427 3 View
For my project, I need to expose zebrafish larvae in petri dish to three concentrations of rotenone (10nM, 30nM and 50nM), and the solutions should be changed daily . My current method was first,...
14 January 2020 4,741 3 View
Actually I already calculated the volume of DMSO I need to add, the question is about the concentration of DMSO. The DMSO in my lab is in liquid form, 100ml, >99.9 %. So I do not know how to...
10 January 2020 3,184 1 View
Anyone can give me some advice in culture THP1 cell line? I have a trouble in thawing THP-1. Why they almost died ? I thaw a vial of THP-1 cell line to the 10 cm dish. The vial contain 10^7cells...
06 January 2020 4,354 3 View
Hi, I am running an MTT assay where i use an acidic solution of isopropanol, HCl and Triton X -100 to dissolve formazan crystals. I have tried using DMSO before but failed to dissolve crystals...
30 December 2019 6,355 2 View
I need help to dissolve my hydrogel beads for antimicrobial test against gram +ve and gram -ve bacteria, I tried with water and DMSO but cant dissolve even with sonication for two hours but not...
27 December 2019 6,687 3 View
After CO2 Electrochemical reduction on 6 samples, i obtained both gaseous and liquid products from GC-MS and NMR respectively. i quantified the liquid products by integrating each peak. for...
15 December 2019 5,801 4 View
Hi, I am performing an MTT assay on HL60 and OCI AML2 cell lines,to detect the IC50 values for cytarabine. I needed to find the appropriate cell seeding density for which i initially seeded 0.1...
04 December 2019 8,994 3 View
Hi, Is it possible to record 13C NMR of stable suspensions in liquid state? I have activated carbon which forms stable dispersion in DMSO. Can I use this suspension to record the 13C NMR in a...
27 November 2019 6,533 2 View
I will use it in whole blood culture. What is the concentration so that it does not affect the cells? Should I perform a cell viability test? They are apolar lipids
19 November 2019 4,800 1 View
I have tried multiple times to cryopreservation of SH-SY5Y cells, but afther thawing, no cells had survived. I bring the cells to cold (4 degrees Celsius) DMEM/F-12 medium with 5% DMSO. After...
15 November 2019 3,732 3 View
Hello all, We are currently working on angiotensin analysis, however we observed that, once the freeze-dried peptide is dissolved in an aqueous buffer, it gets unstable in only 2-3 hours. Mass...
14 November 2019 1,006 1 View
am carrying out a final point pcr from a standardized protocol, but I don't get results. I have modified concentrations of magnesium dichloride, DNTPS, DMSO, change the reaction water, the quality...
07 November 2019 7,985 2 View
My lab recently purchased both the Sigma (F9765) and Cayman (70440) Filipin reagents. These reagents were resuspended in DMSO in the hood in the dark and aliquoted to avoid freeze/thaw. Stock...
06 November 2019 8,617 1 View
Hello, I am trying to encapsulate a hydrophobic drug (that is only soluble in DMSO) in Micelles. The Micelles preparation method is emulsion-solvent evaporation. After mixing the drug/DMSO and...
05 November 2019 1,426 3 View