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Questions related to Cancer Cells
I want to do an experiment on these two types of cells (Hacat and Caco 2). I want to know which one needs more time with trypsin to break the peptide bonds. Thank you
10 October 2017 5,431 3 View
What method do you use to measure the antibody-dependent phagocytosis of cancer cells by macrophages? Do you prefer to use microscopy or flow cytometry for this analysis? Thanks, Chris
06 October 2017 555 3 View
Is it possible to re-infect with Lentivirus cancer cells that were previously infected with other Lentivirus?
04 October 2017 8,208 3 View
In breast cancer, why MDAMB-468 ( epithelial like cells) cells has more lipid content than MDAMB-231 ( messenchymal like cells) cells?
02 October 2017 4,188 1 View
I am doing a viability test to see the application of Calcein AM and EthD-1 on different platforms. Say, I have sub-populations of live and dead MDA-MB-231's in 2.5 mL of ECB (total cell density:...
23 September 2017 1,721 2 View
What is the height of cancer cells growing in 2D cultures? I am mostly interested in human pancreatic BxPC-3 and murine breast cancer 4T1 cells.
23 September 2017 2,184 2 View
Dormancy of the cancer cells results in the recurrence of disease, after treatment of the primary tumor. What might be the possible factors responsible for this phenomena and how these property of...
22 September 2017 7,148 4 View
In a cancer cell, If the majority of genes in fatty acid and cholesterol biosynthesis pathways are upregulated then why cells produce less lipid production?
18 September 2017 4,015 1 View
Dear friends, I am working with a chemical called Allicin. First, Allicin was diluted in NaH2PO4 with PH 4.5 with 1mg/ml and stock in 4oC or below. When I used it for cells (like breast cancer...
14 September 2017 8,234 5 View
A bio active zincoxide nanoparticle sample with -20 zeta potential showed cancer cell growth inhibition. At the same time a bioactive zincsulphide nanoparticle sample with -9 zeta potential...
13 September 2017 3,247 2 View
Lysines are responsible for the regeneration of cells in the body. Their excessive concentration causes the formation of cancer cells. If you control the levels of lysosomes, you could control the...
02 September 2017 6,719 2 View
Does anyone have a protocol for stimulating cancer cells with IFN-gamma to promote CXCL10 production?
28 August 2017 5,519 3 View
HepG2 is considered as a model system for various cytotoxicity experiments according to many studies. Won't it produce wrong results if carcinoma cells have been used for such studies as such...
22 August 2017 5,518 7 View
I'm currently working on a compound that I had isolated which exhibited anticancer activity. It has a therapeutic index of more than 3 and so I'm interested to know its mechanism of action. Based...
04 June 2017 1,067 10 View
I am working on Nuclear Medicine MCNP application. I want to destroy cancerous cells with gamma rays(protons). I am trying to create a source outside the skull and target a beam towards the it. I...
14 May 2017 3,263 3 View
I am working on a compound and studying its cytotoxicity on cancer cells. I want to know if necrosis is the cause of cell death. I would be grateful if someone can suggest how can I study...
08 May 2017 5,695 5 View
Hey Guys, I'm culturing cancerous breast tissue cells in vitro. Today I took the 24 well plate out of the incubator, and, being done with the cells, I sprayed bleach in all 24 wells. Instantly,...
24 April 2017 6,380 1 View
We found that konockdown of a gene didn't cause apoptosis in cancer cells, but its specific inhibitor treatment induced massive apoptosis. How to explain this phenomonon? Could this result be...
11 April 2017 8,533 5 View
Is acrylamide classified as human mutagen or carcinogen? is there any difference between mutagen and carcinogen?
09 April 2017 896 9 View
I'm working on this topic : "CTC enumeration and culture for therapy monitoring through liquid biopsy"the challenge I face is the few number of circulating tumor cell per each ml of blood so I...
06 April 2017 8,222 3 View
This picture explain p53 expression in breast cancer cells?
04 April 2017 7,784 3 View
Hi, I am currently design a assay for testing the cytotoxicity of primary T cells against tumor cell lines. Here is the design: Cryo-preserved PBMCs were retrieved and cultured with 9ug/mL PHA,...
13 February 2017 5,993 2 View
Dear All, I am working on HCT-15 cells,i want to measure the cisplatin uptake,after treated the cells with cisplatin 12h.can any one please tell me the protocol.
02 December 2016 461 3 View
Dear fellow researchers, I have been trying to stain E-cadherin and N-Cadherin in the breast cancer cell in MDA-MB-231 for quite some time. Ideally, they should be expressed at the cell-cell...
28 February 2016 5,258 6 View