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Questions related to Cancer Cells
I'm planning to expose cancer cells to a certain concentration of a substance and am primarily looking to observe its mitotic activity. Does anyone have any suggestions as to what cell assay I can...
12 December 2014 7,328 8 View
Why add penicillin or other antibiotics when culture or keeping cancer cells? Why keep cancer cells in atmosphere of 5% CO2?
12 December 2014 7,646 0 View
How to define a candidate to a proto-oncogene, what kinds of test should be performed.
12 December 2014 6,980 7 View
In order for cancer to spread to distant tissues, metastatic cells must enter the lymph/blood stream. However, this question rises that what will happen if we manually introduce metastatic cancer...
12 December 2014 5,454 1 View
there are studies show reciprocal interaction between cancer cell and stroma mediated by extracellular vesicles ( cancer cell release microvesicles affect stromal cells which in turn release...
12 December 2014 7,438 5 View
http://youtu.be/1hVOdaeET8E time=48 Vit C need to be penetrate insite the cancer cell ---> H2O2---->free radicals of Fe ----> selective destruction of cancer cel (because not has catalase...
11 November 2014 1,196 0 View
I have heard that certain kinds of microorganisms are very sensitive to oxygen and grow only anaerobic conditions. On the contrary, normal, vascularized tissues are hostile environments for such...
11 November 2014 6,504 2 View
PC9 is EGFR mutant non small cell lung cancer cell (adenocarcinoma)
11 November 2014 3,874 4 View
Most of the researchers are wonder about the dual activity of the curcumin.How it is possible for a compound to act as antioxidant(in normal cells) and ROS producer(in cancer cells)?
11 November 2014 5,906 19 View
Is there any study which could explain tankyrase activity in lymphocyte cultures.? I would like to get some general information on tankyrase activity.
11 November 2014 9,384 4 View
Hi everyone, My name is Nguyen Diem and I'm new to cell research. Now I'm working on a biosensor that could measure the secretion of metalloproteinase-9 in cancer cell. I'd like to ask if we can...
11 November 2014 3,323 4 View
It is now a well known fact that, hypoxia is a critical feature of the tumor microenvironment which promotes invasion and metastasis However, in recent years, increasing evidence suggests that...
11 November 2014 9,085 6 View
Please provide some good reference. Where I should inject the cancer cells?
11 November 2014 10,063 4 View
Hi everyone. I am currently working on identification, quantification, statistical analysis and interpretation of metabolites on NMR spectra of a panel of cancer cells. From published articles and...
11 November 2014 6,381 1 View
I have performed 3-5 IF experiments using PP2 in varying concentrations from 3-10 uM and it seems at the higher end of the concentrations I used (5uM and 10uM) half of the cells on my cover slips...
11 November 2014 4,185 2 View
Hi, we are considering use low-temperature gelling agarose to set tumour tissue - in order to then mechanically slice the samples to produce viable groups of tissue/samples of cells- would anyone...
11 November 2014 4,706 1 View
I am working in a lung cancer lab and I am searching for murine lung cancer cells (e.g. lewis lung carcinoma or L1C2) with a stable Ovalbumin-expression (transfection with OVA-expressing...
10 October 2014 9,743 1 View
If yes, then what effect does it impart?
10 October 2014 8,334 3 View
Cancer cells have a combination of aerobic glycolysis and oxidative phosphorylation in producing ATP. Knowing which is the major producer of ATP can give the clinician and idea which pathway can...
10 October 2014 9,438 4 View
We are treating cancer cells with inhibitors to see if we can inhibit the Ras-Raf interaction. I can clearly detect the interaction via coIP but I am having trouble seeing inhibition of this...
10 October 2014 2,134 6 View
I ran a Western blot a few times for the GADD45a gene, but I didn't get clear bands, even when I changed the following conditions: High voltage (100V) to low voltage (80V) Gel concentration from...
10 October 2014 4,911 12 View
I am working on bladder cancer cells. I am using Decitabine drug for my study. For experiments i dissolved drug in DMSO and after that i aliquoted it and stored it in -80. Every time i used a...
10 October 2014 8,015 5 View
In my experiment, I collected condition media from breast cancer cell culture MDA-231 and I added it to normal breast cell to convert it to cancer, what is the advantage when we used condition...
10 October 2014 8,656 6 View
I am working on melanoma exosomes and my interest lies to check if there is any initiation of cancer stem like properties.
10 October 2014 8,475 3 View