I am working on HCT-15 cells,i want to measure the cisplatin uptake,after treated the cells with cisplatin 12h.can any one please tell me the protocol.
Hmm, not sure about that one. Usually people conduct cytotoxicity assays to determine the cell viability after treatment with Cisplatin, I am unaware of actually measuring the amount of Cisplatin that has entered the cell. Sorry I can't be of more help, I am interested to hear what people add to this question.
You can use the radio isotope technology. Initially, you have to make platinum in the form of radio-isotopes. selannjutnya, you will easily calculate how many semi-quantitative levels of platinum.