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Questions related to Cancer Research
1- Sucrose 10% containing protease inhibitors 2-10 mm Tris-HCL, pH = 7.5 and supplemented with the ready-to-use 1× protease inhibitor cocktail 3-Tris buffer (pH = 7.4) containing 10 mM Tris, 10...
23 February 2024 9,664 0 View
Hi, Are there any ways to check for oligo annealing? Thanks, Linoy
24 August 2022 9,486 0 View
I am working with rectal swab samples in the field of colorectal cancer research. Does anyone have experience (or can point towards any relevant literature) of specifically working with proteins...
03 February 2021 3,848 3 View
I read many papers about exosome isolation from fresh blood or frozen plasma, but not for frozen whole blood. I am wondering whether can I get exosomes from cryopreserved whole blood? One of my...
27 October 2020 5,361 3 View
Hello, I am new to cancer research and I am trying to optimize my small cell lung cancer cell protocol. I purchased a cell line from ATCC (H1105) and I have been growing the cells in RPMI 1640...
12 October 2020 5,781 6 View
I am studying on cancer research and ı used bcl 2 antibody to understand there is apoptosis or not. I got good band ı tested with ponceu red. Finally I got this result.ı used uvp chemi doc it2...
20 July 2020 8,843 8 View
recently I am working on a project about the effect of bio-luminescence in cancer research. It would be my honor and pleasure to help me on this way
11 February 2020 7,813 3 View
I currently doing a research for anticancer drugs and have a plan to use CA 15-3 as tumor marker to measure the effectivity. Some reference tell that CA 15-3 and MUC 1 are synonym, but i also...
11 September 2019 2,240 5 View
Cancer research has developed precise biomarkers to use a proper cure for different kind of cancers. Why there is no proper biomarker to find the precise treatment in autoimmune desease, such as...
26 July 2019 9,852 4 View
Since humanized and SCID mice are very expensive to procure and maintain. I was wondering to develop a cost and effective approach to develop an in vivo model for cancer research. Thymectomy is...
11 July 2019 8,253 4 View
Hello! For my study assignment (cancer research, up/downregulation of a gene in high and low density cells) the standard deviation is not necessary but we think it is important to put in our short...
30 March 2019 4,790 7 View
Hi everyone, I plan to perform CAncer Personalized Profiling by deep Sequencing (CAPP-Seq) for a cancer research project. Is there any available genome analysis company that uses Illumina Hiseq...
10 February 2019 5,366 7 View
In cancer research, is it better to study if the modulation of a lncRNA is able to change the tumor size at different times or to see a change in mice survival?
30 May 2018 1,615 2 View
To fight the disease effectively, researchers from across the scientific spectrum and beyond must join forces.
16 April 2018 7,452 33 View
looking for a good replacement as positive control in cancer research
27 February 2018 9,143 3 View
i am working on cancer research on rats. i would like to use antagonist of selective TRP. however, i couldnt find the appropriate dose and the duration of administration of the antagonist on rats....
29 November 2017 6,619 3 View
Hello fellow Patchers and Sealers, I made a pipette solution recently and expected the osmolarity to be around 270 mOsm approximately according to rough calculation...
11 August 2017 9,044 2 View
Hi all. i am studying the anticancer activity of natural pure compound, the IC50 of it is 25µg/mL and the positive control that i was used is 2µg/mL, i was go deep with the compound and i...
11 August 2017 1,815 6 View
Hyper-activation of the HER family receptors, leads to up-regulation of the three vital signaling pathways, such as PI3K-AKT or MAPK pathways. It has been demonstrated that one of the reason for...
25 July 2017 1,549 4 View
Most studies on CAR-T cells use NSG mice (B/T cell deficient) based on the papers I found. Only a few use nude mice (T cell deficient, B cell present), which is what we are hoping to do. Does...
21 July 2017 7,800 3 View
I'm analyzing TCGA data. I'm comparing a gene's expression between tumor and normal. However, it is possible that the patients get any treatment before, which affect the genes expression. How to...
20 July 2017 667 2 View
I need this plasmid to induce p-pg overexpression.
13 July 2017 1,542 1 View
We have an ABI 3730 and we would like to run it on. Of course together with the expanding need for providing BRCA test routinely in our country for a low enough price.
12 July 2017 832 1 View
I read some papers and most of them listed DO-7. Also, what other antibodies are better for p53 detection in terms of quality and reactivity and that are cost effective.
11 July 2017 9,325 6 View