Does anyone have a protocol for stimulating cancer cells with IFN-gamma to promote CXCL10 production?
It depends on the type of the cancer cells you are working on.
Thanks Muthanna, I am working on a murine ovarian cancer cell line called ID8. What would you suggest? Pavlina.
Hi Pavlina, despite the authors have used human ovarian cancer cell lines, I believe the paper below could help you out with setting up your experiments. Authors have tested the ability of increasing concentration of IFN-γ alone or in combiantion with TNF-alpha to stimulate either CXCL9 or CXCL10 in serous ovarian cancer cells. I'd suggest you starting with a similar approach by looking at which concentration works better with your cells and then move on with testing your working hypothesis. 50 ng ml-1 shows a strong to induce CXCL10 in both cell lines and I can tell you that this concentration is often used to activate a variety of immune cell types (e.g. mono/macrophages in culture). Have a look specifically in Fig.4. Hope you're getting out soon results. Best of luck! Damiano
http://www.nature.com/bjc/journal/v115/n5/full/bjc2016172a.html
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