I want to do an experiment on these two types of cells (Hacat and Caco 2). I want to know which one needs more time with trypsin to break the peptide bonds.
Thank you
It is widely known that Caco2 cells are slow and they need weeks to grow , but once they pick up growing they will give a good counting number.Usually, These cells detach eaisly, however if you experience hard time detaching them, I would try using trypsin combined with 2.21 milli molar EDTA.I do not have any information about the other cell line.
HaCaT is a spontaneously transformed aneuploid immortal keratinocyte cell line from adult human skin. It cannot be regarded as cancerous cell line, in fact they are more like immortalised 'normal' non-cancerous cells.
Thank you so much, Ms. Nour Hussein and Ms. Agnieszka Bojko, for your responses.
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