Hey Guys,
I'm culturing cancerous breast tissue cells in vitro. Today I took the 24 well plate out of the incubator, and, being done with the cells, I sprayed bleach in all 24 wells. Instantly, half of the wells turned a deep yellow and the other half did not react (change color) at all. The cells that did react were in normal media where the cells that did not react had metformin (a common diabetes drug) in the media. Has anyone every heard of this? Please help me out even if its just a guess or opinion.
Since clorinated compound present in the bleach react with both phenol red (present in the medium) as well directly on the cells, the color may be changed. Actually higher concentration of bleach may degrade various cells, therefore definitely not good for the cells. Most possibly metformin may directly interact with bleaching and therefore bleaching may not be available to react with phenol red and on the cells.
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