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Questions related to Cancer Cells
I’m working on MDA-MB-231 cells which are breast cancer cells. I changed the medium (L15 medium) of the cells on Thursday afternoon but on Friday I forgot to change it again because weekend was...
02 February 2019 9,814 2 View
I found the information that in order to see cancer cells we need a volume of few mm^3. I wonder how this value may be supported by actual medical data.
31 January 2019 4,435 1 View
I am currently writing an introduction to my thesis and reading "Biology of Cancer" of Weinberg. The book is so awesome and interesting, I just can not express how much I love it: it touches all...
29 January 2019 8,609 5 View
I'm going in depth to find correlation between normal & cancer cell... to find special characteristics which can help us to identify week prognostic profile.
28 January 2019 7,819 2 View
We just identified a mitochondrial protein that regulates fatty acid metabolism (FAM) in cancer cells. Does somebody have expertise in FAM? For example, which kit(s) or method(s) can be used to...
02 January 2019 4,418 3 View
We are culturing primary breast cancer tissues and we are little worried about the medium what we are currently using. We have used advanced DMEM/f12 medium (supplimented with FBS). Is this ok to...
26 December 2018 5,884 2 View
I heard from a colleague that RPP30 is a gene that is very rarely duplicated or deleted in cancer cells, but I haven't been able to find any reference for that. Does anyone know of any research...
17 December 2018 8,580 1 View
Need a protocol for sonicating the Hep3B cells using Ultra-sonication Instrument.
15 December 2018 3,265 4 View
Hi all, may I know how frequent do I need to introduce the cancerous cell (A431) into balb/c mice for tumor development? Or it just need to be done once and wait for it to grow? Thanks
24 November 2018 3,171 3 View
What happens in the cancer cells or diseases to ''attract isotopes''
13 November 2018 7,579 3 View
Hello, I would like to learn the measurement ways for the stemness capacity of the cultured cancer cell. I could not decide what is the best way to do this efficiently. Could anybody recommend...
02 November 2018 3,879 3 View
Weather these enzymes which are involved in Cancer lipid metabolism are the causative genes for the cancers or just they are supplying the required energy for rapid proliferation of cancer cells...?
30 October 2018 5,744 5 View
Can 3D culture be used to differentiate a cell population potentially containing both non cancerous and cancerous cells? If there is a population of cells growing derived from primary tumour...
18 October 2018 379 4 View
We are investigating the effects of plant extracts on human cancer cells. For one of our projects, we want to isolate a certain compound from the extract. We are looking for researchers who could...
15 October 2018 6,012 23 View
Hi, I need to know if Orthovanadate (sodium) can induce expression of STAT3 or increases its phosphorylated form (active STAT3 form). The use of Orthovanadate in lysis buffer to obtained total...
14 October 2018 4,028 2 View
I used 26 gauge needle for subcutaneous injection of matrigel:cancer cells. However, when i pull out the needle, the matrigel solution also came out. How to prevent the leaking?
13 November 2017 5,695 3 View
Why we are not taking T lymphocyte from body of the patient to train it against the patient's cancer cells, cultivate it and inject it back to the patient? Even if this T lymphocyte would be...
13 November 2017 9,901 3 View
Dear All In my case, I have FTIR datasets of cancer cells and cancer cells after treatment. Measuring of them was done in reflectance mode using Nicolet 8700 FTIR Spectrometer with Nicolet...
10 November 2017 7,558 3 View
I want to know that my gold complex will distribute in different parts of cell , cell membrane ,cytoplasm , nucleus etc so what amount of metals goes to each parts of cells, and determine by ICP-MS .
08 November 2017 6,381 1 View
Hello folks! I guess my question might not be quite clear. I am seeking some rare mechanisms that could allow cancer therapeutics take effect outside of the cells. For example, (Maybe) breaking...
05 November 2017 9,844 10 View
I am recently doing PDX model but find serious fibroblast cells contamination. Can anyone share experience on that one?
28 October 2017 4,674 2 View
We use cells as doing subculture (ex: cancer cells). However I don't know of the timing changing the cells. When is the timing changing the cell ?
27 October 2017 4,788 6 View
I'm working in subject related to undestand the mechanisms resistance of cancer cell to drug. For example: Melanoma to Vemurafenib
26 October 2017 8,332 2 View
Hello all, It is clear that cancer cells need to migrate within host tissue during metastasis, but they face the extracellular matrix as a physical barrier. Is it necessary for them to degrade...
25 October 2017 8,451 7 View