What is the height of cancer cells?

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Column for purification of rat monoclonal antibody (kappa chain)?

I have 1L of hybridoma supernatant without serum from a rat monoclonal antibody. I would like to purify it using a binding affinity column for rat kappa chain. Could you please tell me where I...

29 November 2020 3,615 3 View

How to separate over expressed protein?

I have to identify the protein responsible for some activity in microbes and have seen over expressed band in native page. how can i purify it for protein sequencing.

21 October 2019 3,822 2 View

Which one is better: Matrigel or Geltrex?

Has anybody tried practically both substances: Matrigel and Geltrex? I found some literature in the Internet about the differences and similarities between those two substances. Maybe someone...

06 July 2018 1,496 3 View

Is this formulation suitable for the i.p. injection?

I made a formulation which includes 5% dextrose, 10% Cremophor EL and 10% Ethanol (aqueous solution, plus tested compound). It was used for oral administration of compound. Is this compositions...

15 December 2017 7,535 7 View

What is the best tissue clearing solution?

I want to make spheroids more clear for confocal imaging. What are the possible ways to clear them? Maybe somebody tried several different commercial solutions or home-made ones and could suggest...

04 October 2017 2,655 6 View

How to show that ultrasound exists in the cel medium

We are applying ultrasound for live cells grown in multi-well dishes. There is no a noticeable effect on cell viability. How to show that there is an effect of ultrasound at all in the cell medium?

20 July 2017 3,592 2 View

Where is it possible to find a list of certified analytical labs in EU?

Where it would be possible to find a list of accredited testing labs for phamaceuticals in EU (ISO/IEC 17025)?

21 January 2017 4,362 3 View

How can I separate aliphatic and aromatic hydrocarbons by solid phase extraction?

I am looking for a sample preparation technique for total petroleum hydrocarbons that can separate the aliphatic and the aromatic components for GC analysis.

23 October 2014 5,587 10 View

What is the best method for measuring endothelium function?

See above

21 September 2014 541 20 View

Does anyone have any experience with measuring impedance of powder samples (without sintering ceramics)?

Some people used to measure impedance of preыsed powders without sintering a ceramic. How do you prepare electrodes for such samples? The analysis of the data are somehow complicated: sample is...

09 July 2013 5,160 9 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View