I am working on a compound and studying its cytotoxicity on cancer cells. I want to know if necrosis is the cause of cell death.
I would be grateful if someone can suggest how can I study necrosis in cancer cells. Which kits should I use?
Also, apart from kits what can be used to study necrosis signaling cascade, what antibodies could be purchased?
The issue there are several kits and specific proteins available for studying apoptosis but hardly anything more studying necrosis.
Thank you.
Thank you! But can you guys send me references for your comments.
@Venkata reddy: Thanks! But, HIF is also changed in expression in cancers in general. The expression levels of HIF and antioxidants is high in tumors.
@ Margit: Please can you forward me the protocol for the same.
Lastly, just like for apoptosis, we study cleaved PARP, p53 and host of other proteins, can we repeat the same strategy for necrosis?
@Abdul Baig: Thanks, do you have a protocol for the same probes (Trypan blue, 7AAD, PI for flow cytometry)
@Han.....Thanks......what about healthy cells. My assay is as follow..use anticancer drug on cancer cells and then check mechanism of programmed cell death.
can you please forward some literature on the same staining.
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