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Questions related to Cancer Cells
I have to do Annexinv/PI flowcytometry but the prolem is that we have no flowcytometer in our university and I have to take my samples to another university in another city. I have to fix my cells...
09 September 2015 1,062 6 View
I have counted different cell lines like HeLa, LNCap.FGC, PC-3, CHO-b using a haemocytometer on T-25 and T-75 flasks. But even when the flasks are fully confluent, or 90% confluent, I get a very...
09 September 2015 6,029 4 View
Explain difference between cell cycle arrest and apoptosis in cancer cells?
09 September 2015 7,816 5 View
Hi guys, I am recently testing a drug that is supposed to increase the superoxide production in cancer cells. However, I noticed the DHE staining (cytosolic superoxide) increased whereas the...
08 August 2015 5,871 4 View
I am designing a CRISPR based assay and need help designing a positive control. Specifically, can someone tell me a protein which causes cell death when it is expressed in cancer cells ? I prefer...
08 August 2015 1,218 5 View
I need to work on in vitro tumor model that will some what similar to the natural tumor. In the model to develop, macrophage (M2 phage) is very crucial including other cells that generally reside...
08 August 2015 1,984 5 View
Majority of the studies have shown that MCF-7 cells do not express caspase-3, which is however the major apoptotic pathway for a wide range of cancer cells. And if different drugs activate...
08 August 2015 7,478 5 View
Hi, I am doing FISH on cancer cells, my problem is that control signals were visible but not in test signals though I know that test samples are strongly positive. My problem is that if the test...
08 August 2015 7,359 6 View
I want to check the heterogeneous population of cancer cells in a particular tumor.
08 August 2015 7,164 4 View
I am planning to flow breast cancer cells, in a buffer, into a device. What is the highest flow rate I could use if I want the breast cells to attract to the CXCL12 chemokine? Specifically, I am...
07 July 2015 1,380 2 View
Please tell which LPS (strain of E.coli) would give best results. Time of induction? what about starvation condition ? I tried with 100ng/ml to 4 ug/ml but could'nt get COX 2 or cCaspase 1?
06 June 2015 1,540 3 View
Hi, I am now testing a possible EGF inhibitor for cancer cells, but after stimulate the cells with external EGF at 20 and 50 ng/ml the RT-PCR bands instead of increase the density is decreasing...
06 June 2015 986 8 View
There is a Co(III) mustard based prodrug which reduced in hypoxia environment and toxic to the cancer cells. if this complex reduced in cancer cell environment then why not other Co(III)...
06 June 2015 7,759 4 View
I would like to perform a transwell migration assay on breast cancer cells (4T1/410.4/NMuMG) using different pore diameters. Would anyone know if they are too large to pass through a 3um pore? I...
06 June 2015 7,025 2 View
I am using T47Ds, HCC1937s, and SKBR3s. I am plating around 3,000 cells/well in 100 uL in a 96 well plate. I had been told it was not enough to wait 24 hours after plating to treat the cells and...
06 June 2015 946 6 View
Mouse syngenic colon model.
05 May 2015 3,627 8 View
I have use Trizol method to extract my RNA from cancer cells. The A260/280 ratio range is within 1.8-1.9. Would that be possible to increase the value from 1.8 to more than 2 by re-precipitating...
05 May 2015 4,586 6 View
I would like to know about cancer cell differentiation. After inducing differentiation it may reduce tumorigenicity. If somebody can provide reference article and reference drug, that would be...
04 April 2015 2,770 6 View
Thanks in advance for your replies.
03 March 2015 8,548 5 View
I'm working in cancer research and I know that cancer cells have transferrin transporter overexpressed, because they need more iron for celular proliferation. So, I want to know what other metals...
03 March 2015 2,284 11 View
Hello, I am currently working on metastasizing cancer cells to liver via spleen. Here is what I want to ask you guys, the purpose of the experiment is to check how many cancer cells can reach the...
03 March 2015 2,011 5 View
These are chemical compounds present in Rheum ribes and I need papers including in abstract the role of these compounds in inhibiting cancer cells.
03 March 2015 1,171 0 View
Hi all, I am working on cancer cells and my drug shows ROS production and mitochondrial membrane depolarization but I want to check the effect of my drug on mitochondrial respiratory chain complex...
03 March 2015 2,752 10 View
I would like to perform the in vitro synthetic lethality screen in cancer cells to identify the synthetic lethal pair. Is there any HTS method for identification of SL pair? and also any method...
03 March 2015 536 5 View