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Questions related to Cancer Cells
I'm conducting an expirement in Boyden Chamber to see how different tratments will affect MDA-MB-231 cancer cells ability to migrate. Are there any protocols that can help me decide the specific...
03 September 2019 8,482 1 View
I want to see the expression of endothelial secreted molecule in MDA-MB-231 Breast cancer cells. I am curious about the expression whether i should co-culture with endothelial cells Please give...
18 August 2019 8,562 3 View
Is it possible to stock cells after synchronization? Thank you
06 August 2019 9,765 2 View
I need positive control for my wb in intrinsic pathway, especial bcl-2, cytochrome c, survivin and bax
23 July 2019 4,482 2 View
I am planning to do orthotopic injection of breast cancer cells (MDA-MB-231 and 4T1 cells) into the mammary fat pad of mice to observe cancer metastasis and tumor growth. In lots of references I...
05 July 2019 6,649 5 View
Isothermal titration calorimetry is used to measure enthalpy, entropy of any reaction. If we can use the same principle in which the cancer cells will be grown in the test chamber and water in...
19 June 2019 4,092 3 View
When there are only two cells at the beginning, the competition between proliferation and migration may be very remarkable. When a cancer cell is undergoing division, does it slow down/stop...
13 June 2019 2,758 2 View
I am trying to culture MDA MB 231 cells in culture flasks. I need to know if the cells are contaminated by yeast. The cells are base adherent. The color of media is mostly transparent with a shade...
31 May 2019 7,919 4 View
https://www.thermofisher.com/order/catalog/product/L3224 This is the live/dead cell assay kit I will use for my experiment. But I have to fix samples (cancer cells) before using this kit, maybe...
30 April 2019 3,735 3 View
I am treating gold nanoparticles to cells(cancer cells) and see the adhesion & effect of gold nanoparticles on cells. When I look at microscope, I find out that gold nanoparticles are...
11 April 2019 8,946 4 View
What are your recommendations of mounting oil for cancer cell staining , I am looking to stain microtubules and actin in slides then imaging under confocal microscope ? I am using oil...
09 April 2019 4,788 1 View
I want to do colony formation assay using U-87 MG cells, human brain astrocyte tumor. But I have no idea about that. What should I pay attention to?
04 April 2019 9,427 7 View
I have 8 means for 8 concentrations inducing control I calculated the proliferation % How can I statistically analyze if the treatment significantly has an effect or not ?
03 April 2019 2,848 3 View
when designing a chimeric antigen receptor for cart t cell therapy can't we use a target which is also exist in T cells but express excessively in cancerous cells?
03 April 2019 9,016 1 View
I checked 3AO cells online （ATCC ECACC and some company website） could not find this cells‘ background information. eg. ES2 (Morphology)fibroblast (disease)clear cell carcinoma (age)47...
26 March 2019 10,038 1 View
What could be the quantitative amount of H2O2/g of protein present inside the cancer cell?
25 March 2019 2,279 3 View
I am tending to use CAFs in our lab but I do not have them, so I am thinking to expose them to breast cancer cells in order to turn them into CAFs. Any idea please ?
22 March 2019 8,901 3 View
I injected cancer cells to mice as xenografts. After 7 days, I injected tamoxifen together with an inhibitor of interleukin to nude mice subcutaneously. But 2 days later, the tumor site turned to...
22 March 2019 6,380 4 View
The presence of telomerase which prevents telomere shortening in cancer cells is said to be one of the leading causes of the continuous proliferation (avoiding ageing) of cancer cells. What other...
20 March 2019 994 6 View
I'm trying to detect the XIST-RNA expression in Breast cancer cells using the Biotin and Digoxin probes. However, there are always non-specific bindings in my results. I have added RNase-inhibitor...
19 March 2019 4,021 1 View
I am planning an lab study on effect of hypoxia on glucose metabolism of cancer cell. For hypoxia exposure, I am considering anaerobic chamber system. My concern is that if I use this chamber,...
15 March 2019 7,943 8 View
Hello all, I'm relatively new to research and just started growing my own Ovarian cancer cells from a pleural effusion. It's been about 2 weeks now since I've had them in a flask and have...
24 February 2019 6,899 4 View
My research is to study the ginger effect on breast cancer cells. so how to know the best concentration to treat the cells with it to study the effect. Note that i will treat it with different...
17 February 2019 4,698 3 View
Discussion about its results
07 February 2019 4,179 1 View